Comparative genomics and genome biology of <i>Campylobacter showae</i>

  • Tiffany Hsu (Creator)
  • Matthew R Gemmell (Creator)
  • Eric A Franzosa (Creator)
  • Susan Berry (Creator)
  • Indrani Mukhopadhya (Creator)
  • Richard Hansen (Creator)
  • Monia Michaud (Creator)
  • Hans Linde Nielsen (Creator)
  • William G Miller (Creator)
  • Henrik Nielsen (Creator)
  • Mona Bajaj-Elliott (Creator)
  • Curtis Huttenhower (Creator)
  • Wendy S Garrett (Creator)
  • Georgina L Hold (Creator)

Dataset

Description

<i>Campylobacter showae</i> a bacterium historically linked to gingivitis and periodontitis, has recently been associated with inflammatory bowel disease and colorectal cancer. Our aim was to generate genome sequences for new clinical <i>C. showae</i> strains and identify functional properties explaining their pathogenic potential. Eight <i>C. showae</i> genomes were assessed, four strains isolated from inflamed gut tissues from paediatric Crohn’s disease patients, three strains from colonic adenomas, and one from a gastroenteritis patient stool. Genome assemblies were analyzed alongside the only 3 deposited <i>C. showae</i> genomes. The pangenome from these 11 strains consisted of 4686 unique protein families, and the core genome size was estimated at 1050 ± 15 genes with each new genome contributing an additional 206 ± 16 genes. Functional assays indicated that colonic strains segregated into 2 groups: adherent/invasive vs. non-adherent/non-invasive strains. The former possessed Type IV secretion machinery and S-layer proteins, while the latter contained Cas genes and other CRISPR associated proteins. Comparison of gene profiles with strains in Human Microbiome Project metagenomes showed that gut-derived isolates share genes specific to tongue dorsum and supragingival plaque counterparts. Our findings indicate that <i>C. showae</i> strains are phenotypically and genetically diverse and suggest that secretion systems may play an important role in virulence potential.
Date made available2019
PublisherTaylor & Francis
  • Comparative genomics and genome biology of Campylobacter showae

    Hsu, T., Gemmell, M. R., Franzosa, E. A., Berry, S., Mukhopadhya, I., Hansen, R., Michaud, M., Nielsen, H., Miller, W. G., Nielsen, H., Bajaj-Elliott, M., Huttenhower, C., Garrett, W. S. & Hold, G. L., Jun 2019, In: Emerging microbes & infections. 8, 1, p. 827-840 14 p.

    Research output: Contribution to journalJournal articleResearchpeer-review

    Open Access
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