Abstract
A comparison of lignocellulose degradation, energy requirements, biogas production and microbial community analysis was done between a fungal (Pleurotus ostreatus, 28 d, 35 °C), thermal (1 h, 121 °C) and acid (2% HCl, 2 h, 123 °C) pre-treatment of bean straw. The results revealed that the type of pre-treatment had a great impact on lignocellulose degradation and energy demand, where the acid pre-treatment had higher solubilization capacity (lignin 67%, hemicellulose 68% and 0.49 g sugars·g−1 bean straw) and demanded less energy as compared with the fungal pre-treatment. Contrary, the type of pre-treatment did not have an apparent effect on the anaerobic digestion of the hydrolysates after 37 days of bioreactors operation in terms of biogas yield (142–165 mL g−1 chemical oxygen demand-COD), COD and sugars degradation (>80%). The bioreactor using acid pre-treatment hydrolysates showed differences in the total bacterial and archaea estimates, with a more pronounced increase in archaea (Euryarchaeota) and bacteria (Bacteroidetes, Firmicutes, and Proteobacteria), suggesting a more favorable composition for microbial growth. Overall, the acid pre-treatment was the most effective on all the parameters compared in this study. Further studies should include other important parameters to consider when selecting a pre-treatment method including environmental and economic implications of solid phase disposal and pH adjustment.
Original language | English |
---|---|
Article number | 105775 |
Journal | Biomass and Bioenergy |
Volume | 142 |
ISSN | 0961-9534 |
DOIs | |
Publication status | Published - Nov 2020 |
Bibliographical note
Funding Information:This work was financially supported by the grant IPN20150837. J.J. Montoya-Rosales was financially supported by the scholarship IPNBEIFI2124 and the School of Civil Engineering at The University of Queensland.
Publisher Copyright:
© 2020 Elsevier Ltd
Keywords
- Anaerobic digestion
- Bean straw
- Biogas
- Hydrolysate
- Microbial community
- Pre-treatment