A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Kasper Kannegård Karlsen; Anders Hauge Okholm; Jørgen Kjems; Jesper Wengel

doi:10.1016/j.bmc.2013.04.062

A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Kasper Kannegård Karlsen
, Anders Hauge Okholm
, Jørgen Kjems
, Jesper Wengel

Research output: Contribution to journal › Journal article › Research › peer-review

24 Citations (Scopus)

Abstract

A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

Original language	English
Journal	Bioorganic & Medicinal Chemistry
Volume	21
Issue number	20
Pages (from-to)	6186-6190
Number of pages	5
ISSN	0968-0896
DOIs	https://doi.org/10.1016/j.bmc.2013.04.062
Publication status	Published - 1 May 2013
Externally published	Yes

Access to Document

10.1016/j.bmc.2013.04.062

http://www.sciencedirect.com/science/article/pii/S096808961300388X

AUB Link

Search for the material in Aalborg University Library's search engine

Cite this

@article{1eb9c60d3e3f449abcfe7899f2cdade1,

title = "A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)",

abstract = "A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900\%, and detection of RNA in living cells was demonstrated.",

author = "Karlsen, \{Kasper Kanneg{\aa}rd\} and Okholm, \{Anders Hauge\} and J{\o}rgen Kjems and Jesper Wengel",

year = "2013",

month = may,

day = "1",

doi = "10.1016/j.bmc.2013.04.062",

language = "English",

volume = "21",

pages = "6186--6190",

journal = "Bioorganic \& Medicinal Chemistry",

issn = "0968-0896",

publisher = "Elsevier",

number = "20",

}

A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). / Karlsen, Kasper Kannegård; Okholm, Anders Hauge; Kjems, Jørgen et al.
In: Bioorganic & Medicinal Chemistry, Vol. 21, No. 20, 01.05.2013, p. 6186-6190.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

AU - Karlsen, Kasper Kannegård

AU - Okholm, Anders Hauge

AU - Kjems, Jørgen

AU - Wengel, Jesper

PY - 2013/5/1

Y1 - 2013/5/1

N2 - A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

AB - A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

U2 - 10.1016/j.bmc.2013.04.062

DO - 10.1016/j.bmc.2013.04.062

M3 - Journal article

C2 - 23693070

SN - 0968-0896

VL - 21

SP - 6186

EP - 6190

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

IS - 20

ER -

A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Abstract

Access to Document

AUB Link

Fingerprint

Cite this