TY - JOUR
T1 - All Staphylococcus aureus bacteraemia-inducing strains can cause infective endocarditis
T2 - Results of GWAS and experimental animal studies
AU - Bastien, Sylvère
AU - Meyers, Severien
AU - Salgado-Pabón, Wilmara
AU - Giulieri, Stefano G.
AU - Rasigade, Jean-Phillipe
AU - Liesenborghs, Laurens
AU - Kinney, Kyle J.
AU - Couzon, Florence
AU - Martins-Simoes, Patricia
AU - Moing, Vincent Le
AU - Duval, Xavier
AU - Holmes, Natasha E.
AU - Bruun, Niels Eske
AU - Skov, Robert
AU - Howden, Benjamin P.
AU - Fowler Jr., Vance G.
AU - Verhamme, Peter
AU - Andersen, Paal Skytt
AU - Bouchiat, Coralie
AU - Moreau, Karen
AU - Vandenesch, François
N1 - Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
PY - 2023/2
Y1 - 2023/2
N2 - OBJECTIVES: We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB).METHODS: A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves.RESULTS: The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU.CONCLUSION: All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.
AB - OBJECTIVES: We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB).METHODS: A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves.RESULTS: The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU.CONCLUSION: All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.
KW - Animals
KW - Bacteremia/microbiology
KW - Endocarditis, Bacterial/microbiology
KW - Endocarditis/microbiology
KW - Genome-Wide Association Study
KW - Mice
KW - Rabbits
KW - Staphylococcal Infections/microbiology
KW - Staphylococcus aureus/genetics
KW - Bacteraemia
KW - Experimental animal model
KW - Genome-wide association study
KW - Infective endocarditis
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85146001645&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2022.12.028
DO - 10.1016/j.jinf.2022.12.028
M3 - Journal article
C2 - 36603774
SN - 0163-4453
VL - 86
SP - 123
EP - 133
JO - Journal of Infection
JF - Journal of Infection
IS - 2
ER -