Abstract
Purpose: To investigate the presence and concentration of α1-antitrypsin in aqueous humour at the time of corneal rejection and to compare results obtained from patients with reversible and irreversible rejection.
Methods: Samples of aqueous humour were obtained from 17 patients with acute corneal endothelial allograft rejection. The presence of α1-antitrypsin in aqueous humour was confirmed by immunoblotting and measured employing a sandwich ELISA. Total protein concentrations in aqueous humour were measured using Bradford's method. The outcome of corneal rejection episodes was determined 1 month after diagnosing corneal rejection and described as reversible or irreversible rejection.
Results: α1-antitrypsin was detected in aqueous humour. Patients with reversible rejection had significantly higher α1-antitrypsin concentration than patients with irreversible rejection (p = 0.044). There was no significant difference in total protein concentrations (p = 0.745), and no correlation was found between α1-antitrypsin and total protein concentrations (p = 0.368).
Conclusions: α1-antitrypsin in aqueous humour seems to signal a favourable outcome of corneal rejection. The possible mechanism is discussed
Methods: Samples of aqueous humour were obtained from 17 patients with acute corneal endothelial allograft rejection. The presence of α1-antitrypsin in aqueous humour was confirmed by immunoblotting and measured employing a sandwich ELISA. Total protein concentrations in aqueous humour were measured using Bradford's method. The outcome of corneal rejection episodes was determined 1 month after diagnosing corneal rejection and described as reversible or irreversible rejection.
Results: α1-antitrypsin was detected in aqueous humour. Patients with reversible rejection had significantly higher α1-antitrypsin concentration than patients with irreversible rejection (p = 0.044). There was no significant difference in total protein concentrations (p = 0.745), and no correlation was found between α1-antitrypsin and total protein concentrations (p = 0.368).
Conclusions: α1-antitrypsin in aqueous humour seems to signal a favourable outcome of corneal rejection. The possible mechanism is discussed
| Original language | English |
|---|---|
| Journal | Acta Ophthalmologica Scandinavica |
| Volume | 83 |
| Issue number | 3 |
| Pages (from-to) | 379-384 |
| Number of pages | 6 |
| ISSN | 1395-3907 |
| DOIs | |
| Publication status | Published - Jun 2005 |
| Externally published | Yes |
Keywords
- cornea rejection immune