Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites

Trine Sørensen; Celine Petersen; Asmus T. Muurmann; Johan V. Christiansen; Mathias L. Brundtø; Christina K. Overgaard; Anders T. Boysen; Rasmus D. Wollenberg; Thomas O. Larsen; Jens L. Sørensen; Kåre L. Nielsen; Teis E. Sondergaard

doi:10.1186/s43008-024-00141-0

Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites

Trine Sørensen, Celine Petersen, Asmus T. Muurmann, Johan V. Christiansen, Mathias L. Brundtø, Christina K. Overgaard, Anders T. Boysen, Rasmus D. Wollenberg, Thomas O. Larsen, Jens L. Sørensen, Kåre L. Nielsen^*, Teis E. Sondergaard^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.

Original language	English
Article number	10
Journal	IMA Fungus
Volume	15
Issue number	1
ISSN	2210-6340
DOIs	https://doi.org/10.1186/s43008-024-00141-0
Publication status	Published - 7 Apr 2024

Bibliographical note

Publisher Copyright:
© The Author(s) 2024.

Keywords

Apiospora
Arthrinium
CAZymes
Oxford Nanopore sequencing
Secondary metabolites

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Sørensen, T., Petersen, C., Muurmann, A. T., Christiansen, J. V., Brundtø, M. L., Overgaard, C. K., Boysen, A. T., Wollenberg, R. D., Larsen, T. O., Sørensen, J. L., Nielsen, K. L., & Sondergaard, T. E. (2024). Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites. IMA Fungus, 15(1), Article 10. https://doi.org/10.1186/s43008-024-00141-0

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title = "Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites",

abstract = "The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.",

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doi = "10.1186/s43008-024-00141-0",

language = "English",

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AU - Christiansen, Johan V.

AU - Brundtø, Mathias L.

AU - Overgaard, Christina K.

AU - Boysen, Anders T.

AU - Wollenberg, Rasmus D.

AU - Larsen, Thomas O.

AU - Sørensen, Jens L.

AU - Nielsen, Kåre L.

AU - Sondergaard, Teis E.

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AB - The Apiospora genus comprises filamentous fungi with promising potential, though its full capabilities remain undiscovered. In this study, we present the first genome assembly of an Apiospora arundinis isolate, demonstrating a highly complete and contiguous assembly estimated to 48.8 Mb, with an N99 of 3.0 Mb. Our analysis predicted a total of 15,725 genes, with functional annotations for 13,619 of them, revealing a fungus capable of producing very high amounts of carbohydrate-active enzymes (CAZymes) and secondary metabolites. Through transcriptomic analysis, we observed differential gene expression in response to varying growth media, with several genes related to carbohydrate metabolism showing significant upregulation when the fungus was cultivated on a hay-based medium. Finally, our metabolomic analysis unveiled a fungus capable of producing a diverse array of metabolites.

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KW - CAZymes

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Apiospora arundinis, a panoply of carbohydrate-active enzymes and secondary metabolites

Abstract

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Keywords

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