TY - JOUR
T1 - Assessing labelled carbon assimilation from poly butylene adipate-co-terephthalate (PBAT) monomers during thermophilic anaerobic digestion
AU - Poulsen, Jan Struckmann
AU - Trueba-Santiso, Alba
AU - Lema, Juan
AU - Echers, Simon Gregersen
AU - Wimmer, Reinhard
AU - Nielsen, Jeppe Lund
PY - 2023/10
Y1 - 2023/10
N2 - PBAT (poly butylene adipate-co-terephthalate) is a widely used biodegradable plastic, but the knowledge about its metabolization in anaerobic environments is very limited. In this study, the anaerobic digester sludge from a municipal wastewater treatment plant was used as inoculum to investigate the biodegradability of PBAT monomers in thermophilic conditions. The research employs a combination of 13C-labelled monomers and proteogenomics to track the labelled carbon and identify the microorganisms involved. A total of 122 labelled peptides of interest were identified for adipic acid (AA) and 1,4-butanedio (BD). Through the time-dependent isotopic enrichment and isotopic profile distributions, Bacteroides, Ichthyobacterium, and Methanosarcina were proven to be directly involved in the metabolization of at least one monomer. This study provides a first insight into the identity and genomic potential of microorganisms responsible for biodegradability of PBAT monomers during anaerobic digestion under thermophilic conditions.
AB - PBAT (poly butylene adipate-co-terephthalate) is a widely used biodegradable plastic, but the knowledge about its metabolization in anaerobic environments is very limited. In this study, the anaerobic digester sludge from a municipal wastewater treatment plant was used as inoculum to investigate the biodegradability of PBAT monomers in thermophilic conditions. The research employs a combination of 13C-labelled monomers and proteogenomics to track the labelled carbon and identify the microorganisms involved. A total of 122 labelled peptides of interest were identified for adipic acid (AA) and 1,4-butanedio (BD). Through the time-dependent isotopic enrichment and isotopic profile distributions, Bacteroides, Ichthyobacterium, and Methanosarcina were proven to be directly involved in the metabolization of at least one monomer. This study provides a first insight into the identity and genomic potential of microorganisms responsible for biodegradability of PBAT monomers during anaerobic digestion under thermophilic conditions.
KW - Anaerobic biodegradation
KW - Isotope labelling
KW - PBAT
KW - Plastic
KW - Proteogenomics
UR - http://www.scopus.com/inward/record.url?scp=85164432178&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2023.129430
DO - 10.1016/j.biortech.2023.129430
M3 - Journal article
SN - 0960-8524
VL - 385
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 129430
ER -