Assigning Function to Phylogeny: MAR-FISH

Jeppe L. Nielsen*

*Corresponding author for this work

Research output: Contribution to book/anthology/report/conference proceedingBook chapterResearchpeer-review

Abstract

Microautoradiography (MAR) is a technique by which assimilated radioactive tracers incorporated into the biomass can be detected by a film emulsion. This allows for the testing of cellular preferences in electron donors and acceptors of individual cells in complex microbial assemblages, as well as the ability to take up substrates under diverse environmental exposures. Combination with staining techniques such as fluorescence in situ hybridization (FISH) can be used to identify the involved cells. Here, the practical aspects of a combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) approach are described.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages12
Volume2246
Place of PublicationNew York
PublisherHumana Press, Inc.
Publication date2021
Pages225-236
ISBN (Print)978-1-0716-1114-2
ISBN (Electronic)Online ISBN 978-1-0716-1115-9
DOIs
Publication statusPublished - 2021
SeriesMethods in Molecular Biology
Volume2246
ISSN1064-3745

Keywords

  • Ecophysiology
  • MAR-FISH
  • Microautoradiography
  • Microbial ecology
  • Single cell imaging

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