Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain

George Nabin Baroi, Ivan Baumann, Peter Westermann, Hariklia N. Gavala

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

Abstract

Only little research on butyric acid fermentation has been carried out in relationship to bio-refinery perspectives involving strain selection, development of adapted strains, physiological analyses for higher yield, productivity and selectivity. However, a major step towards the development of a sustainable industrial society is a shift from petroleum-based resources to the use of renewable resources. An ongoing effort is focused on developing bio-refineries as an alternative way of producing fuels and chemical building-blocks from renewable resources. Thus, today’s organic waste may become tomorrow’s platform for a variety of products for industrial use. Butyric acid is considered as a potential chemical building-block for the production of chemicals for e.g. polymeric compounds and the aim of this work was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces less acetic acid (higher selectivity), has a higher yield and a higher productivity of butyric acid from pre-treated lignocellulosic biomass. Pre-treated wheat straw was used as the main carbon source. After one year of serial adaptation and selection a mutant strain of C. tyrobutyricum was developed. This new strain could grow in up to 80% pre-treated wheat straw and can ferment both glucose and xylose. The yield of butyric acid without optimization was 0,37±0,051 g butyric acid/g sugar monomers and the acetate yield was 0,06±0,021 g acetic acid/g sugar monomers. Moreover, the strain could grow without addition of yeast extract. Further optimization of yield and productivity is under investigation.
Original languageEnglish
Publication date5 Jun 2013
Publication statusPublished - 5 Jun 2013
Event9th International Conference on Renewable Resources and Biorefineries - Antwerp, Belgium
Duration: 5 Jun 20137 Jun 2013
Conference number: 9

Conference

Conference9th International Conference on Renewable Resources and Biorefineries
Number9
CountryBelgium
CityAntwerp
Period05/06/201307/06/2013

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Clostridium tyrobutyricum
butyric acid
wheat straw
fermentation
mutants
renewable resources
acetic acid
sugars
organic wastes
yeast extract
xylose
sustainable development
petroleum
acetates
glucose
carbon
biomass

Cite this

Baroi, G. N., Baumann, I., Westermann, P., & Gavala, H. N. (2013). Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain. Abstract from 9th International Conference on Renewable Resources and Biorefineries , Antwerp, Belgium.
Baroi, George Nabin ; Baumann, Ivan ; Westermann, Peter ; Gavala, Hariklia N. / Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain. Abstract from 9th International Conference on Renewable Resources and Biorefineries , Antwerp, Belgium.
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Baroi, GN, Baumann, I, Westermann, P & Gavala, HN 2013, 'Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain' 9th International Conference on Renewable Resources and Biorefineries , Antwerp, Belgium, 05/06/2013 - 07/06/2013, .

Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain. / Baroi, George Nabin; Baumann, Ivan; Westermann, Peter; Gavala, Hariklia N.

2013. Abstract from 9th International Conference on Renewable Resources and Biorefineries , Antwerp, Belgium.

Research output: Contribution to conference without publisher/journalConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain

AU - Baroi, George Nabin

AU - Baumann, Ivan

AU - Westermann, Peter

AU - Gavala, Hariklia N.

PY - 2013/6/5

Y1 - 2013/6/5

N2 - Only little research on butyric acid fermentation has been carried out in relationship to bio-refinery perspectives involving strain selection, development of adapted strains, physiological analyses for higher yield, productivity and selectivity. However, a major step towards the development of a sustainable industrial society is a shift from petroleum-based resources to the use of renewable resources. An ongoing effort is focused on developing bio-refineries as an alternative way of producing fuels and chemical building-blocks from renewable resources. Thus, today’s organic waste may become tomorrow’s platform for a variety of products for industrial use. Butyric acid is considered as a potential chemical building-block for the production of chemicals for e.g. polymeric compounds and the aim of this work was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces less acetic acid (higher selectivity), has a higher yield and a higher productivity of butyric acid from pre-treated lignocellulosic biomass. Pre-treated wheat straw was used as the main carbon source. After one year of serial adaptation and selection a mutant strain of C. tyrobutyricum was developed. This new strain could grow in up to 80% pre-treated wheat straw and can ferment both glucose and xylose. The yield of butyric acid without optimization was 0,37±0,051 g butyric acid/g sugar monomers and the acetate yield was 0,06±0,021 g acetic acid/g sugar monomers. Moreover, the strain could grow without addition of yeast extract. Further optimization of yield and productivity is under investigation.

AB - Only little research on butyric acid fermentation has been carried out in relationship to bio-refinery perspectives involving strain selection, development of adapted strains, physiological analyses for higher yield, productivity and selectivity. However, a major step towards the development of a sustainable industrial society is a shift from petroleum-based resources to the use of renewable resources. An ongoing effort is focused on developing bio-refineries as an alternative way of producing fuels and chemical building-blocks from renewable resources. Thus, today’s organic waste may become tomorrow’s platform for a variety of products for industrial use. Butyric acid is considered as a potential chemical building-block for the production of chemicals for e.g. polymeric compounds and the aim of this work was to develop a suitable and robust strain of Clostridium tyrobutyricum that produces less acetic acid (higher selectivity), has a higher yield and a higher productivity of butyric acid from pre-treated lignocellulosic biomass. Pre-treated wheat straw was used as the main carbon source. After one year of serial adaptation and selection a mutant strain of C. tyrobutyricum was developed. This new strain could grow in up to 80% pre-treated wheat straw and can ferment both glucose and xylose. The yield of butyric acid without optimization was 0,37±0,051 g butyric acid/g sugar monomers and the acetate yield was 0,06±0,021 g acetic acid/g sugar monomers. Moreover, the strain could grow without addition of yeast extract. Further optimization of yield and productivity is under investigation.

M3 - Conference abstract for conference

ER -

Baroi GN, Baumann I, Westermann P, Gavala HN. Butyric acid fermentation from pre-treated wheat straw by a mutant clostridium tyrobutyricum strain. 2013. Abstract from 9th International Conference on Renewable Resources and Biorefineries , Antwerp, Belgium.