Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum

George Nabin Baroi, Peter Westermann, Hariklia N. Gavala

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

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Abstract

Butyric acid fermentation has long been discussed in the last decade due to the wide application of butyric acid in chemical, pharmaceutical and food industries. Among other microbial strains, C.tyrobutyricum was found interesting due to its higher yield (more than 93% of the theoretical yield) and higher productivity. However, very few studies have focused on fermentation of sugars derived from lignocellulogic biomass. The present study investigates butyric acid fermentation from pretreated and enzymatically hydrolyzed wheat straw. The sugars derived from wheat straw consist mainly of glucose and xylose at a concentration of 71,6±0,2 g/l and 55,4±0,2 g/l respectively, with TS content 20,87% (g/g). From an economical point of view, the conversion of both sugars is very important. In fact C.tyrobutyricum has the capability to convert both hexose and pentose sugars. Results from batch experiments with C.tyrobutyricum showed that the strain was capable of producing butyric acid from hydrolyzed wheat straw by simultaneous uptake of glucose and xylose. Maximum yield of butyric acid (0,47g/g sugars, 96% of the theoretical yield) was obtained at a pH between 6 and 7 with higher selectivity (>90%). However, the xylose uptake rate was much lower than that of glucose, implying that further process development is required. Moreover, reduced cell growth rate was observed at higher metal (K+) concentration added during pH control which could be overcome by in-situ separation of butyric acid.
Original languageEnglish
Publication date12 Sep 2013
Publication statusPublished - 12 Sep 2013
EventInternational Conference on Industrial biotechnology – meeting the challenges - Medicon Village, Lund, Sweden
Duration: 12 Sep 201313 Sep 2013

Conference

ConferenceInternational Conference on Industrial biotechnology – meeting the challenges
LocationMedicon Village
CountrySweden
CityLund
Period12/09/201313/09/2013

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butyric acid
wheat straw
fermentation
sugars
xylose
glucose
uptake mechanisms
chemical industry
pentoses
hexoses
food industry
cell growth
metals
biomass

Cite this

Baroi, G. N., Westermann, P., & Gavala, H. N. (2013). Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum. Poster presented at International Conference on Industrial biotechnology – meeting the challenges, Lund, Sweden.
Baroi, George Nabin ; Westermann, Peter ; Gavala, Hariklia N. / Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum. Poster presented at International Conference on Industrial biotechnology – meeting the challenges, Lund, Sweden.
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abstract = "Butyric acid fermentation has long been discussed in the last decade due to the wide application of butyric acid in chemical, pharmaceutical and food industries. Among other microbial strains, C.tyrobutyricum was found interesting due to its higher yield (more than 93{\%} of the theoretical yield) and higher productivity. However, very few studies have focused on fermentation of sugars derived from lignocellulogic biomass. The present study investigates butyric acid fermentation from pretreated and enzymatically hydrolyzed wheat straw. The sugars derived from wheat straw consist mainly of glucose and xylose at a concentration of 71,6±0,2 g/l and 55,4±0,2 g/l respectively, with TS content 20,87{\%} (g/g). From an economical point of view, the conversion of both sugars is very important. In fact C.tyrobutyricum has the capability to convert both hexose and pentose sugars. Results from batch experiments with C.tyrobutyricum showed that the strain was capable of producing butyric acid from hydrolyzed wheat straw by simultaneous uptake of glucose and xylose. Maximum yield of butyric acid (0,47g/g sugars, 96{\%} of the theoretical yield) was obtained at a pH between 6 and 7 with higher selectivity (>90{\%}). However, the xylose uptake rate was much lower than that of glucose, implying that further process development is required. Moreover, reduced cell growth rate was observed at higher metal (K+) concentration added during pH control which could be overcome by in-situ separation of butyric acid.",
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Baroi, GN, Westermann, P & Gavala, HN 2013, 'Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum' International Conference on Industrial biotechnology – meeting the challenges, Lund, Sweden, 12/09/2013 - 13/09/2013, .

Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum. / Baroi, George Nabin; Westermann, Peter; Gavala, Hariklia N.

2013. Poster presented at International Conference on Industrial biotechnology – meeting the challenges, Lund, Sweden.

Research output: Contribution to conference without publisher/journalPosterResearchpeer-review

TY - CONF

T1 - Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum

AU - Baroi, George Nabin

AU - Westermann, Peter

AU - Gavala, Hariklia N.

PY - 2013/9/12

Y1 - 2013/9/12

N2 - Butyric acid fermentation has long been discussed in the last decade due to the wide application of butyric acid in chemical, pharmaceutical and food industries. Among other microbial strains, C.tyrobutyricum was found interesting due to its higher yield (more than 93% of the theoretical yield) and higher productivity. However, very few studies have focused on fermentation of sugars derived from lignocellulogic biomass. The present study investigates butyric acid fermentation from pretreated and enzymatically hydrolyzed wheat straw. The sugars derived from wheat straw consist mainly of glucose and xylose at a concentration of 71,6±0,2 g/l and 55,4±0,2 g/l respectively, with TS content 20,87% (g/g). From an economical point of view, the conversion of both sugars is very important. In fact C.tyrobutyricum has the capability to convert both hexose and pentose sugars. Results from batch experiments with C.tyrobutyricum showed that the strain was capable of producing butyric acid from hydrolyzed wheat straw by simultaneous uptake of glucose and xylose. Maximum yield of butyric acid (0,47g/g sugars, 96% of the theoretical yield) was obtained at a pH between 6 and 7 with higher selectivity (>90%). However, the xylose uptake rate was much lower than that of glucose, implying that further process development is required. Moreover, reduced cell growth rate was observed at higher metal (K+) concentration added during pH control which could be overcome by in-situ separation of butyric acid.

AB - Butyric acid fermentation has long been discussed in the last decade due to the wide application of butyric acid in chemical, pharmaceutical and food industries. Among other microbial strains, C.tyrobutyricum was found interesting due to its higher yield (more than 93% of the theoretical yield) and higher productivity. However, very few studies have focused on fermentation of sugars derived from lignocellulogic biomass. The present study investigates butyric acid fermentation from pretreated and enzymatically hydrolyzed wheat straw. The sugars derived from wheat straw consist mainly of glucose and xylose at a concentration of 71,6±0,2 g/l and 55,4±0,2 g/l respectively, with TS content 20,87% (g/g). From an economical point of view, the conversion of both sugars is very important. In fact C.tyrobutyricum has the capability to convert both hexose and pentose sugars. Results from batch experiments with C.tyrobutyricum showed that the strain was capable of producing butyric acid from hydrolyzed wheat straw by simultaneous uptake of glucose and xylose. Maximum yield of butyric acid (0,47g/g sugars, 96% of the theoretical yield) was obtained at a pH between 6 and 7 with higher selectivity (>90%). However, the xylose uptake rate was much lower than that of glucose, implying that further process development is required. Moreover, reduced cell growth rate was observed at higher metal (K+) concentration added during pH control which could be overcome by in-situ separation of butyric acid.

M3 - Poster

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Baroi GN, Westermann P, Gavala HN. Butyric acid fermentation from pretreated and hydrolyzed wheat straw by C.tyrobutyricum. 2013. Poster presented at International Conference on Industrial biotechnology – meeting the challenges, Lund, Sweden.