Caught in the act: discovering secreted proteins from fungi and oomycetes in action

Doris Roth, Morten Nedergaard Grell, Annette Bruun Jensen, Thomas Sundelin, Daniel Buchvaldt Amby, Fredrik Heyman, Tomas Johansson, Jørgen Eilenberg, Birgit Jensen, Dan Funck Jensen, Anders Tunlid, Lene Lange

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

Host-microbe relationships largely rely on secreted proteins like enzymes, virulence factors and antimicrobial peptides. To discover proteins secreted by microbe and host during the interaction with each other, we produced dual-organism cDNA libraries from three different fungus- or oomycete-infected host systems. By methods such as TAST (transposon-assisted signal trapping) and PCR with degenerated primers, the libraries are screened for secreted proteins, especially enzymes, which are then further characterized. We investigate a fourth system, the mycorrhizal fungus Paxillus convolutus, by applying a similar strategy with a fungus-only library. As a result, we will show that our approach is widely applicable and allows us to deepen the understanding a variety of different host-microbe systems.

Original languageEnglish
Publication date2010
Publication statusPublished - 2010
Event10th European Conference on Fungal Genetics - Noordwijkerhout, Netherlands
Duration: 29 Mar 20101 Apr 2010

Conference

Conference10th European Conference on Fungal Genetics
CountryNetherlands
CityNoordwijkerhout
Period29/03/201001/04/2010

Cite this

Roth, D., Grell, M. N., Jensen, A. B., Sundelin, T., Amby, D. B., Heyman, F., ... Lange, L. (2010). Caught in the act: discovering secreted proteins from fungi and oomycetes in action. Poster session presented at 10th European Conference on Fungal Genetics, Noordwijkerhout, Netherlands.
Roth, Doris ; Grell, Morten Nedergaard ; Jensen, Annette Bruun ; Sundelin, Thomas ; Amby, Daniel Buchvaldt ; Heyman, Fredrik ; Johansson, Tomas ; Eilenberg, Jørgen ; Jensen, Birgit ; Jensen, Dan Funck ; Tunlid, Anders ; Lange, Lene. / Caught in the act: discovering secreted proteins from fungi and oomycetes in action. Poster session presented at 10th European Conference on Fungal Genetics, Noordwijkerhout, Netherlands.
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title = "Caught in the act: discovering secreted proteins from fungi and oomycetes in action",
abstract = "Host-microbe relationships largely rely on secreted proteins like enzymes, virulence factors and antimicrobial peptides. To discover proteins secreted by microbe and host during the interaction with each other, we produced dual-organism cDNA libraries from three different fungus- or oomycete-infected host systems. By methods such as TAST (transposon-assisted signal trapping) and PCR with degenerated primers, the libraries are screened for secreted proteins, especially enzymes, which are then further characterized. We investigate a fourth system, the mycorrhizal fungus Paxillus convolutus, by applying a similar strategy with a fungus-only library. As a result, we will show that our approach is widely applicable and allows us to deepen the understanding a variety of different host-microbe systems.",
author = "Doris Roth and Grell, {Morten Nedergaard} and Jensen, {Annette Bruun} and Thomas Sundelin and Amby, {Daniel Buchvaldt} and Fredrik Heyman and Tomas Johansson and J{\o}rgen Eilenberg and Birgit Jensen and Jensen, {Dan Funck} and Anders Tunlid and Lene Lange",
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language = "English",
note = "null ; Conference date: 29-03-2010 Through 01-04-2010",

}

Roth, D, Grell, MN, Jensen, AB, Sundelin, T, Amby, DB, Heyman, F, Johansson, T, Eilenberg, J, Jensen, B, Jensen, DF, Tunlid, A & Lange, L 2010, 'Caught in the act: discovering secreted proteins from fungi and oomycetes in action', Noordwijkerhout, Netherlands, 29/03/2010 - 01/04/2010, .

Caught in the act: discovering secreted proteins from fungi and oomycetes in action. / Roth, Doris; Grell, Morten Nedergaard; Jensen, Annette Bruun; Sundelin, Thomas; Amby, Daniel Buchvaldt; Heyman, Fredrik; Johansson, Tomas; Eilenberg, Jørgen; Jensen, Birgit; Jensen, Dan Funck; Tunlid, Anders; Lange, Lene.

2010. Poster session presented at 10th European Conference on Fungal Genetics, Noordwijkerhout, Netherlands.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Caught in the act: discovering secreted proteins from fungi and oomycetes in action

AU - Roth, Doris

AU - Grell, Morten Nedergaard

AU - Jensen, Annette Bruun

AU - Sundelin, Thomas

AU - Amby, Daniel Buchvaldt

AU - Heyman, Fredrik

AU - Johansson, Tomas

AU - Eilenberg, Jørgen

AU - Jensen, Birgit

AU - Jensen, Dan Funck

AU - Tunlid, Anders

AU - Lange, Lene

PY - 2010

Y1 - 2010

N2 - Host-microbe relationships largely rely on secreted proteins like enzymes, virulence factors and antimicrobial peptides. To discover proteins secreted by microbe and host during the interaction with each other, we produced dual-organism cDNA libraries from three different fungus- or oomycete-infected host systems. By methods such as TAST (transposon-assisted signal trapping) and PCR with degenerated primers, the libraries are screened for secreted proteins, especially enzymes, which are then further characterized. We investigate a fourth system, the mycorrhizal fungus Paxillus convolutus, by applying a similar strategy with a fungus-only library. As a result, we will show that our approach is widely applicable and allows us to deepen the understanding a variety of different host-microbe systems.

AB - Host-microbe relationships largely rely on secreted proteins like enzymes, virulence factors and antimicrobial peptides. To discover proteins secreted by microbe and host during the interaction with each other, we produced dual-organism cDNA libraries from three different fungus- or oomycete-infected host systems. By methods such as TAST (transposon-assisted signal trapping) and PCR with degenerated primers, the libraries are screened for secreted proteins, especially enzymes, which are then further characterized. We investigate a fourth system, the mycorrhizal fungus Paxillus convolutus, by applying a similar strategy with a fungus-only library. As a result, we will show that our approach is widely applicable and allows us to deepen the understanding a variety of different host-microbe systems.

M3 - Poster

ER -

Roth D, Grell MN, Jensen AB, Sundelin T, Amby DB, Heyman F et al. Caught in the act: discovering secreted proteins from fungi and oomycetes in action. 2010. Poster session presented at 10th European Conference on Fungal Genetics, Noordwijkerhout, Netherlands.