TY - JOUR
T1 - Characterisation of cellulose-degrading organisms in an anaerobic digester
AU - Poulsen, Jan Struckmann
AU - Jonge, Nadieh de
AU - Macedo, Williane Vieira
AU - Rask Dalby, Frederik
AU - Feilberg, Anders
AU - Lund Nielsen, Jeppe
PY - 2022/5
Y1 - 2022/5
N2 - The recalcitrant nature of lignocellulosic biomass hinders efficient exploitation of this fraction for energy production. A better understanding of the microorganisms able to convert plant-based feedstocks is needed to improve anaerobic digestion of lignocellulosic biomass. In this study, active thermophilic cellulose-degrading microorganisms were identified from a full-scale anaerobic digester fed with maize by using metagenome-resolved protein stable isotope probing (protein-SIP). 13C-cellulose was converted into 13C-methane with a 13/12C isotope ratio of 0.127 after two days of incubation. Metagenomic analysis revealed 238 different genes coding for carbohydrate-active enzymes (CAZymes), six of which were directly associated with cellulose degradation. The protein-SIP analysis identified twenty heavily labelled peptides deriving from microorganisms actively assimilating labelled carbon from the degradation of 13C-cellulose, highlighting several members of the order Clostridiales. Corynebacterium was identified through CAZyme screening, amplicon analysis, and in the metagenome giving a strong identification of being a cellulose degrader.
AB - The recalcitrant nature of lignocellulosic biomass hinders efficient exploitation of this fraction for energy production. A better understanding of the microorganisms able to convert plant-based feedstocks is needed to improve anaerobic digestion of lignocellulosic biomass. In this study, active thermophilic cellulose-degrading microorganisms were identified from a full-scale anaerobic digester fed with maize by using metagenome-resolved protein stable isotope probing (protein-SIP). 13C-cellulose was converted into 13C-methane with a 13/12C isotope ratio of 0.127 after two days of incubation. Metagenomic analysis revealed 238 different genes coding for carbohydrate-active enzymes (CAZymes), six of which were directly associated with cellulose degradation. The protein-SIP analysis identified twenty heavily labelled peptides deriving from microorganisms actively assimilating labelled carbon from the degradation of 13C-cellulose, highlighting several members of the order Clostridiales. Corynebacterium was identified through CAZyme screening, amplicon analysis, and in the metagenome giving a strong identification of being a cellulose degrader.
KW - Anaerobic digestion
KW - Lignocellulosic biomass
KW - Metaproteomics
KW - Microbial community
KW - Protein stable isotope probing
UR - http://www.scopus.com/inward/record.url?scp=85126136085&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2022.126933
DO - 10.1016/j.biortech.2022.126933
M3 - Journal article
SN - 0960-8524
VL - 351
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 126933
ER -