Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa; Raluca G Maltesen; Bodil S Rasmussen; Katrine B Buggeskov; Hanne B Ravn; Martin Skott; Søren Nielsen; Jørgen Frøkiær; Troels Ring; Reinhard Wimmer

doi:10.1007/s11306-019-1629-8

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa, Raluca G Maltesen, Bodil S Rasmussen, Katrine B Buggeskov, Hanne B Ravn, Martin Skott, Søren Nielsen, Jørgen Frøkiær, Troels Ring, Reinhard Wimmer

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.

AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.

METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.

RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.

CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

Original language	English
Article number	7
Journal	Metabolomics
Volume	16
Issue number	1
Number of pages	7
ISSN	1573-3882
DOIs	https://doi.org/10.1007/s11306-019-1629-8
Publication status	Published - 1 Jan 2020

Keywords

Metabolomics
NMR
Plasma
Reproducibility
Serum

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title = "Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra",

abstract = "BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.",

keywords = "Metabolomics, NMR, Plasma, Reproducibility, Serum",

author = "Hanifa, {Munsoor A} and Maltesen, {Raluca G} and Rasmussen, {Bodil S} and Buggeskov, {Katrine B} and Ravn, {Hanne B} and Martin Skott and S{\o}ren Nielsen and J{\o}rgen Fr{\o}ki{\ae}r and Troels Ring and Reinhard Wimmer",

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doi = "10.1007/s11306-019-1629-8",

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T1 - Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

AU - Hanifa, Munsoor A

AU - Maltesen, Raluca G

AU - Rasmussen, Bodil S

AU - Buggeskov, Katrine B

AU - Ravn, Hanne B

AU - Skott, Martin

AU - Nielsen, Søren

AU - Frøkiær, Jørgen

AU - Ring, Troels

AU - Wimmer, Reinhard

PY - 2020/1/1

Y1 - 2020/1/1

N2 - BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

AB - BACKGROUND: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.AIMS: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.METHODS: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.RESULTS: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.CONCLUSIONS: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

KW - Metabolomics

KW - NMR

KW - Plasma

KW - Reproducibility

KW - Serum

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U2 - 10.1007/s11306-019-1629-8

DO - 10.1007/s11306-019-1629-8

M3 - Journal article

C2 - 31858270

SN - 1573-3882

VL - 16

JO - Metabolomics

JF - Metabolomics

IS - 1

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ER -

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Abstract

Keywords

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NMR-Based Metabolomics in Kidney and Lung Disease: investigating metabolite biomarkers in a rat chronic kidney disease model and in human lung injury

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