Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples

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Abstract

Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described.
Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30 min. The protein content of the samples was characterized by high throughput quantitative proteomics.
We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor differences in protein quantitation.
Original languageEnglish
JournalEuPA Open Proteomics
Volume10
Pages (from-to)9-18
ISSN2212-9685
DOIs
Publication statusPublished - 2016

Keywords

  • proteomics
  • RNAlater
  • paraffin-embedded
  • human colon mucosa
  • preservation
  • mass spectrometry

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