Abstract
Cripto-1 is a protein participating in tissue orientation during embryogenesis but has also been implicated in a wide variety of cancers, such as colon, lung and breast cancer. Cripto-1 plays a role in the regulation of different pathways, including TGF-β/Smad and Wnt/β-catenin, which are highly associated with cell migration both during embryonal development and cancer progression. Little is known about the detailed subcellular localization of cripto-1 and how it participates in the directional movement of cells. In this study, the subcellular localization of cripto-1 in glioblastoma cells was investigated in vitro with high-resolution microscopy techniques. Cripto-1 was found to be localized to dynamic and shed filopodia and transported between cells through tunneling nanotubes. Our results connect the refined subcellular localization of cripto-1 to its functions in cellular orientation and migration.
| Original language | English |
|---|---|
| Article number | 151044 |
| Journal | European Journal of Cell Biology |
| Volume | 98 |
| Issue number | 5-8 |
| ISSN | 0171-9335 |
| DOIs | |
| Publication status | Published - Dec 2019 |
Keywords
- CD44
- Cripto-1
- Dynamics
- EV
- Filopodia
- Glioblastoma
- In vitro
- Retraction fibers
- Secretion
- Subcellular
- Tunneling nanotubes
- Vesicles