Dechloromonas: to be or not to be a PAO? That is the question!

Francesca Petriglieri; Caitlin Margaret Singleton; Miriam Peces Gomez; Jette Fischer Petersen; Marta Anna Nierychlo; Per Halkjær Nielsen

Dechloromonas: to be or not to be a PAO? That is the question!

Francesca Petriglieri, Caitlin Margaret Singleton, Miriam Peces Gomez, Jette Fischer Petersen, Marta Anna Nierychlo, Per Halkjær Nielsen

Research output: Contribution to conference without publisher/journal › Poster › Research

127 Downloads (Pure)

Abstract

Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

Original language	English
Publication date	18 Nov 2019
Publication status	Published - 18 Nov 2019
Event	8th IWA Microbial Ecology and Water Engineering Specialist Conference - Hiroshima, Japan Duration: 17 Nov 2019 → 20 Nov 2019 https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/

Conference

Conference	8th IWA Microbial Ecology and Water Engineering Specialist Conference
Country/Territory	Japan
City	Hiroshima
Period	17/11/2019 → 20/11/2019
Internet address	https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/

Keywords

Dechloromonas
PAO
Raman-FISH
P mass-balance

Access to Document

Dechloromonas: to be or not to be a PAO? That is the question!

AUB Link

Search for the material in Aalborg University Library's search engine

Cite this

@conference{e495c305ffde4ea590b7e6f258c310f0,

title = "Dechloromonas: to be or not to be a PAO? That is the question!",

abstract = "Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.",

keywords = "Dechloromonas, PAO, Raman-FISH, P mass-balance",

author = "Francesca Petriglieri and Singleton, {Caitlin Margaret} and Gomez, {Miriam Peces} and Petersen, {Jette Fischer} and Nierychlo, {Marta Anna} and Nielsen, {Per Halkj{\ae}r}",

year = "2019",

month = nov,

day = "18",

language = "English",

note = "8th IWA Microbial Ecology and Water Engineering Specialist Conference ; Conference date: 17-11-2019 Through 20-11-2019",

url = "https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/",

}

TY - CONF

T1 - Dechloromonas: to be or not to be a PAO? That is the question!

AU - Petriglieri, Francesca

AU - Singleton, Caitlin Margaret

AU - Gomez, Miriam Peces

AU - Petersen, Jette Fischer

AU - Nierychlo, Marta Anna

AU - Nielsen, Per Halkjær

PY - 2019/11/18

Y1 - 2019/11/18

N2 - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

AB - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

KW - Dechloromonas

KW - PAO

KW - Raman-FISH

KW - P mass-balance

M3 - Poster

T2 - 8th IWA Microbial Ecology and Water Engineering Specialist Conference

Y2 - 17 November 2019 through 20 November 2019

ER -

Dechloromonas: to be or not to be a PAO? That is the question!

Abstract

Conference

Keywords

Access to Document

AUB Link

Fingerprint

Cite this