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Abstract

Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.
Original languageEnglish
Publication date18 Nov 2019
Publication statusPublished - 18 Nov 2019
Event8th IWA Microbial Ecology and Water Engineering Specialist Conference - Hiroshima, Japan
Duration: 17 Nov 201920 Nov 2019
https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/

Conference

Conference8th IWA Microbial Ecology and Water Engineering Specialist Conference
CountryJapan
CityHiroshima
Period17/11/201920/11/2019
Internet address

Fingerprint

Dechloromonas
famine
activated sludge
cells
glycogen
polymers
phenotype
genome

Keywords

  • Dechloromonas
  • PAO
  • Raman-FISH
  • P mass-balance

Cite this

Petriglieri, F., Singleton, C. M., Gomez, M. P., Petersen, J. F., Nierychlo, M. A., & Nielsen, P. H. (2019). Dechloromonas: to be or not to be a PAO? That is the question!. Poster presented at 8th IWA Microbial Ecology and Water Engineering Specialist Conference, Hiroshima, Japan.
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title = "Dechloromonas: to be or not to be a PAO? That is the question!",
abstract = "Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2{\%} complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.",
keywords = "Dechloromonas, PAO, Raman-FISH, P mass-balance",
author = "Francesca Petriglieri and Singleton, {Caitlin Margaret} and Gomez, {Miriam Peces} and Petersen, {Jette Fischer} and Nierychlo, {Marta Anna} and Nielsen, {Per Halkj{\ae}r}",
year = "2019",
month = "11",
day = "18",
language = "English",
note = "null ; Conference date: 17-11-2019 Through 20-11-2019",
url = "https://iwa-network.org/events/microbial-ecology-and-water-engineering-specialist-group-conference/",

}

Petriglieri, F, Singleton, CM, Gomez, MP, Petersen, JF, Nierychlo, MA & Nielsen, PH 2019, 'Dechloromonas: to be or not to be a PAO? That is the question!', 8th IWA Microbial Ecology and Water Engineering Specialist Conference, Hiroshima, Japan, 17/11/2019 - 20/11/2019.

Dechloromonas: to be or not to be a PAO? That is the question! / Petriglieri, Francesca; Singleton, Caitlin Margaret; Gomez, Miriam Peces; Petersen, Jette Fischer; Nierychlo, Marta Anna; Nielsen, Per Halkjær.

2019. Poster presented at 8th IWA Microbial Ecology and Water Engineering Specialist Conference, Hiroshima, Japan.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Dechloromonas: to be or not to be a PAO? That is the question!

AU - Petriglieri, Francesca

AU - Singleton, Caitlin Margaret

AU - Gomez, Miriam Peces

AU - Petersen, Jette Fischer

AU - Nierychlo, Marta Anna

AU - Nielsen, Per Halkjær

PY - 2019/11/18

Y1 - 2019/11/18

N2 - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

AB - Members of the genus Dechloromonas are often abundant in EBPR plants worldwide and have long been considered putative PAOs, as intracellular poly-P has been identified with traditional staining methods. This study applied metagenomics to determine the metabolic potential and Raman microspectroscopy to verify and define the levels and dynamics of important storage polymers in FISH-defined Dechloromonas cells. A 99.2% complete metagenome-assembled genome, retrieved from Danish EBPR plants, showed the potential for poly-P, glycogen, and PHA accumulation. Dechloromonas cells exhibited in situ a Ca. Accumulibacter-like phenotype with dynamic levels of poly-P and PHA during feast-famine cycling. Quantitative Raman-FISH analysis of single cells from full-scale activated sludge plants confirmed Dechloromonas to be an important PAO.

KW - Dechloromonas

KW - PAO

KW - Raman-FISH

KW - P mass-balance

M3 - Poster

ER -

Petriglieri F, Singleton CM, Gomez MP, Petersen JF, Nierychlo MA, Nielsen PH. Dechloromonas: to be or not to be a PAO? That is the question!. 2019. Poster presented at 8th IWA Microbial Ecology and Water Engineering Specialist Conference, Hiroshima, Japan.