Detection of increased pyruvate dehydrogenase flux in the human heart during adenosine stress test using hyperpolarized [1-13C]pyruvate cardiovascular magnetic resonance imaging

Steen Hylgaard Joergensen*, Esben Soevsoe S. Hansen, Nikolaj Bøgh, Lotte Bonde Bertelsen, Peter Bisgaard Staehr, Rolf F. Schulte, Craig Malloy, Henrik Wiggers, Christoffer Laustsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

12 Citations (Scopus)
26 Downloads (Pure)

Abstract

BACKGROUND: Hyperpolarized (HP) [1-13C]pyruvate cardiovascular magnetic resonance (CMR) imaging can visualize the uptake and intracellular conversion of [1-13C]pyruvate to either [1-13C]lactate or 13C-bicarbonate depending on the prevailing metabolic state. The aim of the present study was to combine an adenosine stress test with HP [1-13C]pyruvate CMR to detect cardiac metabolism in the healthy human heart at rest and during moderate stress.

METHODS: A prospective descriptive study was performed between October 2019 and August 2020. Healthy human subjects underwent cine CMR and HP [1-13C]pyruvate CMR at rest and during adenosine stress. HP [1-13C]pyruvate CMR images were acquired at the mid-left-ventricle (LV) level. Semi-quantitative assessment of first-pass myocardial [1-13C]pyruvate perfusion and metabolism were assessed. Paired t-tests were used to compare mean values at rest and during stress.

RESULTS: Six healthy subjects (two female), age 29 ± 7 years were studied and no adverse reactions occurred. Myocardial [1-13C]pyruvate perfusion was significantly increased during stress with a reduction in time-to-peak from 6.2 ± 2.8 to 2.7 ± 1.3 s, p = 0.02. This higher perfusion was accompanied by an overall increased myocardial uptake and metabolism. The conversion rate constant (kPL) for lactate increased from 11 ± 9 *10-3 to 20 ± 10 * 10-3 s-1, p = 0.04. The pyruvate oxidation rate (kPB) increased from 4 ± 4 *10-3 to 12 ± 7 *10-3 s-1, p = 0.008. This increase in carbohydrate metabolism was positively correlated with heart rate (R2 = 0.44, p = 0.02).

CONCLUSIONS: Adenosine stress testing combined with HP [1-13C]pyruvate CMR is feasible and well-tolerated in healthy subjects. We observed an increased pyruvate oxidation during cardiac stress. The present study is an important step in the translation of HP [1-13C]pyruvate CMR into clinical cardiac imaging. Trial registration EUDRACT, 2018-003533-15. Registered 4th of December 2018, https://www.clinicaltrialsregister.eu/ctr-search/search?query=2018-003533-15.

Original languageEnglish
Article number34
JournalJournal of Cardiovascular Magnetic Resonance
Volume24
Issue number1
ISSN1097-6647
DOIs
Publication statusPublished - Dec 2022

Bibliographical note

© 2022. The Author(s).

Keywords

  • Adenosine
  • Adult
  • Exercise Test
  • Female
  • Humans
  • Lactates
  • Magnetic Resonance Imaging/methods
  • Magnetic Resonance Imaging, Cine
  • Male
  • Myocardial Perfusion Imaging/methods
  • Oxidoreductases
  • Predictive Value of Tests
  • Prospective Studies
  • Pyruvic Acid
  • Young Adult
  • Cardiac metabolism
  • Perfusion
  • Metabolic imaging
  • Stress test

Fingerprint

Dive into the research topics of 'Detection of increased pyruvate dehydrogenase flux in the human heart during adenosine stress test using hyperpolarized [1-13C]pyruvate cardiovascular magnetic resonance imaging'. Together they form a unique fingerprint.

Cite this