Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene

Kazumi Miyazaki; Christophe K Mapendano; Tomokazu Fuchigami; Shinji Kondo; Tohru Ohta; Akira Kinoshita; Kazuhiro Tsukamoto; Ko-ichiro Yoshiura; Norio Niikawa; Tatsuya Kishino

doi:10.1016/j.gene.2008.11.019

Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene

Kazumi Miyazaki, Christophe K Mapendano, Tomokazu Fuchigami, Shinji Kondo, Tohru Ohta, Akira Kinoshita, Kazuhiro Tsukamoto, Ko-ichiro Yoshiura, Norio Niikawa, Tatsuya Kishino

Research output: Contribution to journal › Journal article › Research › peer-review

13 Citations (Scopus)

Abstract

The mouse Snurf/Snrpn gene has two differentially methylated regions (DMRs), the maternally methylated region at the 5' end (DMR1) and the paternally methylated region at the 3' end (DMR2). DMR1, a region that includes the Snrpn promoter and the entire intron 1, has been thought to be a germline DMR, which inherits the parental-specific methylation profile from the gametes. DMR1 is not only associated with imprinted Snrpn expression, but implicated in imprinting control of other genes in the region. We have now characterized the highly conserved activator sequence (CAS) in the Snrpn intron 1 among human and rodents and demonstrate that the mouse CAS is not a germline DMR but shows developmentally dynamic changes of DNA methylation and has methylation-sensitive enhancer activity. The tissue-specific methylation of the mouse CAS and its methylation-sensitive enhancer activity may control tissue-specific expression of IC transcripts, resulting in the establishment and/or maintenance of imprinting in the Snrpn locus.

Original language	English
Journal	Gene
Volume	432
Issue number	1-2
Pages (from-to)	97-101
Number of pages	5
ISSN	0378-1119
DOIs	https://doi.org/10.1016/j.gene.2008.11.019
Publication status	Published - 2009
Externally published	Yes

Keywords

Alleles
Animals
Brain/metabolism
Cells, Cultured
Conserved Sequence
CpG Islands/genetics
DNA Methylation
Enhancer Elements, Genetic
Female
Gene Expression Regulation, Developmental
Genome/genetics
Humans
Lymphocytes/metabolism
Male
Mice
Neurons/metabolism
Organ Specificity
snRNP Core Proteins/genetics

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@article{a5e45f04c6b44c68b299a4f91d893477,

title = "Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene",

abstract = "The mouse Snurf/Snrpn gene has two differentially methylated regions (DMRs), the maternally methylated region at the 5' end (DMR1) and the paternally methylated region at the 3' end (DMR2). DMR1, a region that includes the Snrpn promoter and the entire intron 1, has been thought to be a germline DMR, which inherits the parental-specific methylation profile from the gametes. DMR1 is not only associated with imprinted Snrpn expression, but implicated in imprinting control of other genes in the region. We have now characterized the highly conserved activator sequence (CAS) in the Snrpn intron 1 among human and rodents and demonstrate that the mouse CAS is not a germline DMR but shows developmentally dynamic changes of DNA methylation and has methylation-sensitive enhancer activity. The tissue-specific methylation of the mouse CAS and its methylation-sensitive enhancer activity may control tissue-specific expression of IC transcripts, resulting in the establishment and/or maintenance of imprinting in the Snrpn locus.",

keywords = "Alleles, Animals, Brain/metabolism, Cells, Cultured, Conserved Sequence, CpG Islands/genetics, DNA Methylation, Enhancer Elements, Genetic, Female, Gene Expression Regulation, Developmental, Genome/genetics, Humans, Lymphocytes/metabolism, Male, Mice, Neurons/metabolism, Organ Specificity, snRNP Core Proteins/genetics",

author = "Kazumi Miyazaki and Mapendano, {Christophe K} and Tomokazu Fuchigami and Shinji Kondo and Tohru Ohta and Akira Kinoshita and Kazuhiro Tsukamoto and Ko-ichiro Yoshiura and Norio Niikawa and Tatsuya Kishino",

year = "2009",

doi = "10.1016/j.gene.2008.11.019",

language = "English",

volume = "432",

pages = "97--101",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene

AU - Miyazaki, Kazumi

AU - Mapendano, Christophe K

AU - Fuchigami, Tomokazu

AU - Kondo, Shinji

AU - Ohta, Tohru

AU - Kinoshita, Akira

AU - Tsukamoto, Kazuhiro

AU - Yoshiura, Ko-ichiro

AU - Niikawa, Norio

AU - Kishino, Tatsuya

PY - 2009

Y1 - 2009

N2 - The mouse Snurf/Snrpn gene has two differentially methylated regions (DMRs), the maternally methylated region at the 5' end (DMR1) and the paternally methylated region at the 3' end (DMR2). DMR1, a region that includes the Snrpn promoter and the entire intron 1, has been thought to be a germline DMR, which inherits the parental-specific methylation profile from the gametes. DMR1 is not only associated with imprinted Snrpn expression, but implicated in imprinting control of other genes in the region. We have now characterized the highly conserved activator sequence (CAS) in the Snrpn intron 1 among human and rodents and demonstrate that the mouse CAS is not a germline DMR but shows developmentally dynamic changes of DNA methylation and has methylation-sensitive enhancer activity. The tissue-specific methylation of the mouse CAS and its methylation-sensitive enhancer activity may control tissue-specific expression of IC transcripts, resulting in the establishment and/or maintenance of imprinting in the Snrpn locus.

AB - The mouse Snurf/Snrpn gene has two differentially methylated regions (DMRs), the maternally methylated region at the 5' end (DMR1) and the paternally methylated region at the 3' end (DMR2). DMR1, a region that includes the Snrpn promoter and the entire intron 1, has been thought to be a germline DMR, which inherits the parental-specific methylation profile from the gametes. DMR1 is not only associated with imprinted Snrpn expression, but implicated in imprinting control of other genes in the region. We have now characterized the highly conserved activator sequence (CAS) in the Snrpn intron 1 among human and rodents and demonstrate that the mouse CAS is not a germline DMR but shows developmentally dynamic changes of DNA methylation and has methylation-sensitive enhancer activity. The tissue-specific methylation of the mouse CAS and its methylation-sensitive enhancer activity may control tissue-specific expression of IC transcripts, resulting in the establishment and/or maintenance of imprinting in the Snrpn locus.

KW - Alleles

KW - Animals

KW - Brain/metabolism

KW - Cells, Cultured

KW - Conserved Sequence

KW - CpG Islands/genetics

KW - DNA Methylation

KW - Enhancer Elements, Genetic

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Genome/genetics

KW - Humans

KW - Lymphocytes/metabolism

KW - Male

KW - Mice

KW - Neurons/metabolism

KW - Organ Specificity

KW - snRNP Core Proteins/genetics

U2 - 10.1016/j.gene.2008.11.019

DO - 10.1016/j.gene.2008.11.019

M3 - Journal article

C2 - 19095049

SN - 0378-1119

VL - 432

SP - 97

EP - 101

JO - Gene

JF - Gene

IS - 1-2

ER -

Developmentally dynamic changes of DNA methylation in the mouse Snurf/Snrpn gene

Abstract

Keywords

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