Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Zivile Kondrotaite; Laura C. Valk; Francesca Petriglieri; Caitlin Singleton; Marta Nierychlo; Morten K.D. Dueholm; Per H. Nielsen

doi:10.3389/fmicb.2022.917553

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Zivile Kondrotaite, Laura C. Valk, Francesca Petriglieri, Caitlin Singleton, Marta Nierychlo, Morten K.D. Dueholm, Per H. Nielsen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of Saprospiraceae. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence in situ hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in Candidatus Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These results provide the first overview of the most abundant novel Saprospiraceae genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.

Original language	English
Article number	917553
Journal	Frontiers in Microbiology
Volume	13
ISSN	1664-302X
DOIs	https://doi.org/10.3389/fmicb.2022.917553
Publication status	Published - 8 Jul 2022

Bibliographical note

Funding Information:
This project was funded by the Villum Foundation (Dark Matter grant13351) and Aalborg University.

Publisher Copyright:
Copyright © 2022 Kondrotaite, Valk, Petriglieri, Singleton, Nierychlo, Dueholm and Nielsen.

Keywords

activated sludge
FISH–Raman
high-quality MAGs
MiDAS
OLB8
Saprospiraceae
wastewater

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@article{a580901143af4ea8a85f45b7f7e66d8c,

title = "Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems",

abstract = "The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of Saprospiraceae. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence in situ hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in Candidatus Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These results provide the first overview of the most abundant novel Saprospiraceae genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.",

keywords = "activated sludge, FISH–Raman, high-quality MAGs, MiDAS, OLB8, Saprospiraceae, wastewater",

author = "Zivile Kondrotaite and Valk, {Laura C.} and Francesca Petriglieri and Caitlin Singleton and Marta Nierychlo and Dueholm, {Morten K.D.} and Nielsen, {Per H.}",

note = "Funding Information: This project was funded by the Villum Foundation (Dark Matter grant13351) and Aalborg University. Publisher Copyright: Copyright {\textcopyright} 2022 Kondrotaite, Valk, Petriglieri, Singleton, Nierychlo, Dueholm and Nielsen.",

year = "2022",

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day = "8",

doi = "10.3389/fmicb.2022.917553",

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T1 - Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

AU - Kondrotaite, Zivile

AU - Valk, Laura C.

AU - Petriglieri, Francesca

AU - Singleton, Caitlin

AU - Nierychlo, Marta

AU - Dueholm, Morten K.D.

AU - Nielsen, Per H.

N1 - Funding Information: This project was funded by the Villum Foundation (Dark Matter grant13351) and Aalborg University. Publisher Copyright: Copyright © 2022 Kondrotaite, Valk, Petriglieri, Singleton, Nierychlo, Dueholm and Nielsen.

PY - 2022/7/8

Y1 - 2022/7/8

N2 - The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of Saprospiraceae. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence in situ hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in Candidatus Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These results provide the first overview of the most abundant novel Saprospiraceae genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.

AB - The Saprospiraceae family within the phylum Bacteroidota is commonly present and highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is known about its role. In this study, we used MiDAS 4 global survey with samples from 30 countries to analyze the abundance and distribution of members of Saprospiraceae. Phylogenomics were used to delineate five new genera from a set of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed probes for fluorescence in situ hybridization (FISH) revealed rod-shaped morphologies for all genera analyzed, including OLB8, present mostly inside the activated sludge flocs. The genomes revealed potential metabolic capabilities for the degradation of polysaccharides, proteins, and other complex molecules; partial denitrification; and storage of intracellular polymers (glycogen, polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman microspectroscopy confirmed the presence of intracellular glycogen in Candidatus Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These results provide the first overview of the most abundant novel Saprospiraceae genera present in WWTPs across the world and their potential involvement in nutrient removal and the degradation of macromolecules.

KW - activated sludge

KW - FISH–Raman

KW - high-quality MAGs

KW - MiDAS

KW - OLB8

KW - Saprospiraceae

KW - wastewater

UR - http://www.scopus.com/inward/record.url?scp=85134528020&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2022.917553

DO - 10.3389/fmicb.2022.917553

M3 - Journal article

AN - SCOPUS:85134528020

SN - 1664-302X

VL - 13

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 917553

ER -

Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured Saprospiraceae Genera in Global Wastewater Treatment Systems

Abstract

Bibliographical note

Keywords

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