Diversity and physiology of uncultured and abundant OLB8 genus and other genera in Saprospiraceae family across global wastewater treatment systems

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Abstract

The Saprospiraceae family is a very diverse family within the phylum Bacteroidetes,
commonly present in high abundance in wastewater treatment plants (WWTPs) worldwide,
such as the genus OLB8, but little is known about their function and importance. The genera
described are characterized by rod-shaped or filamentous morphology and an aerobic
heterotrophic metabolism with their potential involvement in nutrient removal in WWTPs.
Here, we used the MiDAS4 global survey, including samples from 480 WWTPs located in 30
countries, to analyze the abundance and global distribution of members of the Saprospiraceae
family. In addition, we retrieved 32 high-quality metagenome assembled genomes (MAGs)
from Danish WWTPs for metabolic reconstruction, and designed novel fluorescence in situ
hybridization (FISH) probes for visualization and in combination with Raman
microspectroscopy to detect and confirm important physiological features. FISH revealed rodshaped morphologies for the three most abundant genera, present mostly inside the flocs. The
genomic potential reveled diverse metabolism for genus OLB8 and other genera with possible
degradation of polysaccharides, other carbon complexes and partial denitrification. FISHRaman showed a presence of glycogen and PHA, also confirmed by the metabolic potential of
MAGs, while none of the genera contained polyphosphate. These results provide the first
overview of some of the most abundant Saprospiraceae genera present across the world (Figure
1) and their potential involvement in polysaccharides and other carbon complex degradation
and nutrient removal in WWTPs.
Original languageEnglish
Publication date2021
Publication statusPublished - 2021
EventThe 9th Microbial Ecology & Water Engineering Specialist Conference -
Duration: 18 Oct 202120 Oct 2021

Conference

ConferenceThe 9th Microbial Ecology & Water Engineering Specialist Conference
Period18/10/202120/10/2021

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