eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in Cyprus

K. Grammatiki; N. de Jonge; J. L. Nielsen; S. C. García-Gomez; E. Avramidi; M. M. Lymperaki; M. Marcou; G. Ioannou; M. Papatheodoulou; O. Dargent; D. Xevgenos; M. Hesselsøe; F. C. Küpper

doi:10.1016/j.marpolbul.2025.117609

eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in Cyprus

K. Grammatiki, N. de Jonge, J. L. Nielsen, S. C. García-Gomez, E. Avramidi, M. M. Lymperaki, M. Marcou, G. Ioannou, M. Papatheodoulou, O. Dargent, D. Xevgenos, M. Hesselsøe, F. C. Küpper^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world – including Cyprus and the wider Mediterranean – Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.

Original language	English
Article number	117609
Journal	Marine Pollution Bulletin
Volume	214
Number of pages	13
ISSN	0025-326X
DOIs	https://doi.org/10.1016/j.marpolbul.2025.117609
Publication status	Published - May 2025

Bibliographical note

Publisher Copyright:
© 2025 The Authors

Keywords

Brine
Cyprus
Desalination
Environmental impact
Marine invertebrates
Metabarcoding

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10.1016/j.marpolbul.2025.117609Licence: CC BY 4.0

Open Access articleFinal published version, 3.1 MBLicence: CC BY 4.0

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Grammatiki, K., de Jonge, N., Nielsen, J. L., García-Gomez, S. C., Avramidi, E., Lymperaki, M. M., Marcou, M., Ioannou, G., Papatheodoulou, M., Dargent, O., Xevgenos, D., Hesselsøe, M., & Küpper, F. C. (2025). eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in Cyprus. Marine Pollution Bulletin, 214, Article 117609. https://doi.org/10.1016/j.marpolbul.2025.117609

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abstract = "Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world – including Cyprus and the wider Mediterranean – Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.",

keywords = "Brine, Cyprus, Desalination, Environmental impact, Marine invertebrates, Metabarcoding",

author = "K. Grammatiki and {de Jonge}, N. and Nielsen, {J. L.} and Garc{\'i}a-Gomez, {S. C.} and E. Avramidi and Lymperaki, {M. M.} and M. Marcou and G. Ioannou and M. Papatheodoulou and O. Dargent and D. Xevgenos and M. Hessels{\o}e and K{\"u}pper, {F. C.}",

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year = "2025",

month = may,

doi = "10.1016/j.marpolbul.2025.117609",

language = "English",

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Grammatiki, K, de Jonge, N , Nielsen, JL, García-Gomez, SC, Avramidi, E, Lymperaki, MM, Marcou, M, Ioannou, G, Papatheodoulou, M, Dargent, O, Xevgenos, D, Hesselsøe, M & Küpper, FC 2025, 'eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in Cyprus', Marine Pollution Bulletin, vol. 214, 117609. https://doi.org/10.1016/j.marpolbul.2025.117609

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AU - Grammatiki, K.

AU - de Jonge, N.

AU - Nielsen, J. L.

AU - García-Gomez, S. C.

AU - Avramidi, E.

AU - Lymperaki, M. M.

AU - Marcou, M.

AU - Ioannou, G.

AU - Papatheodoulou, M.

AU - Dargent, O.

AU - Xevgenos, D.

AU - Hesselsøe, M.

AU - Küpper, F. C.

PY - 2025/5

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N2 - Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world – including Cyprus and the wider Mediterranean – Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.

AB - Seawater desalination has become an essential part of the drinking water supply of many arid countries in the world – including Cyprus and the wider Mediterranean – Middle Eastern region. In this study, the microinvertebrate fauna near the outfall sites of 2 seawater desalination plants, Dhekelia and Larnaca, in Cyprus was assessed by microscopy-based classical taxonomy and eDNA metabarcoding. The results show impact of desalination brine discharge on marine benthic communities. The community structure and the impact of abiotic factors was more prominent in the sediments than the water, and the alpha diversity results from metabarcoding were consistent with the results from microscopy but differed taxonomically.

KW - Brine

KW - Cyprus

KW - Desalination

KW - Environmental impact

KW - Marine invertebrates

KW - Metabarcoding

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DO - 10.1016/j.marpolbul.2025.117609

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AN - SCOPUS:86000475366

SN - 0025-326X

VL - 214

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

M1 - 117609

ER -

eDNA metabarcoding of marine invertebrate communities at RO desalination plant outfalls in Cyprus

Abstract

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