Effect of environmental pH on sperm kinematic characteristics

Hiva Alipour, Fereshteh Dardmeh, Marianne Charlotte Dissing, H. Ingolf Nielsen

Research output: Contribution to book/anthology/report/conference proceedingConference abstract in proceedingResearch

Abstract

Semen preparation medium have an important role in assisted reproduction techniques and their composition influences sperm binding and motility.
Some studies have assessed the influence of pH on sperm kinetics. However, no study to date has assessed the effect of environmental pH on subtle differences in the details of the sperm movement (kinematics) of human sperm provided by computerized sperm analysis systems. This study was designed to assess the effect of two different media pH levels on kinematic parameters of the human sperm.
Samples were prepared using the 40%/80% Pureception (Sage, USA) density gradient and resuspended in customized sperm culture media with pH levels of 7.9 and 8.3 (Origio, Denmark). Kinematic parameters of the sperm in both groups were analyzed using the Sperm Class Analyzer (Microptic S.L., Spain) at 0, 6 and 24 hours following the addition of media.
Results of this study illustrated a general insignificant decrease in the ratio of progressively motile and hyperactive sperm after 6 and 24 hours. However a significant difference between the test groups was observed in the curvilinear, straight line and Mean path velocity and Straightness after 6 and 24 hours. Linearity and Wobble showed significant difference after 24 hours.
This study demonstrated a difference in the sperm motion pattern and velocity in different environmental pH levels. Based on these findings, further investigations are required to elucidate knowledge about possible effect of marginal pH changes, affecting the sperm motion characteristics at different stages in the dynamic environment of the female reproductive tract.
Original languageEnglish
Title of host publicationFinal programme, 18th World Congress of In Vitro Ferilization, ISIVF 2015, 27-30 September 2015, Copenhagen, Denmark
PublisherInternational Society of In Vitro Fertilization
Publication dateSep 2015
Pages22, No. P16
Publication statusPublished - Sep 2015
Event The World Congress Of In Vitro Fertilization - ISIVF 2015 - Copenhagen, Denmark
Duration: 27 Sep 201530 Sep 2015
Conference number: 18

Conference

Conference The World Congress Of In Vitro Fertilization - ISIVF 2015
Number18
Country/TerritoryDenmark
CityCopenhagen
Period27/09/201530/09/2015

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