Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures

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2 Citations (Scopus)

Abstract

Background
A modification of the Flexcell system that allows imposition of homogenous, controlled non-equibiaxial strains to cell cultures is developed and experimentally validated. The Flexcell system by default applies equibiaxial strain to cell cultures, meaning no shear strain, while soft tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis.

Method
The Flexcell system was redesigned using a finite element model in order to obtain large areas of the membrane in a controlled, uniform non-equibiaxial strain state.

Results
The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain.

Conclusion
The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions.

Original languageEnglish
JournalJournal of Tissue Viability
Volume22
Issue number2
Pages (from-to)52-56
Number of pages5
ISSN0965-206X
DOIs
Publication statusPublished - May 2013

Cite this

@article{475e63e7963541bdb16aee979a1f27f4,
title = "Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures",
abstract = "Background A modification of the Flexcell system that allows imposition of homogenous, controlled non-equibiaxial strains to cell cultures is developed and experimentally validated. The Flexcell system by default applies equibiaxial strain to cell cultures, meaning no shear strain, while soft tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis. Method The Flexcell system was redesigned using a finite element model in order to obtain large areas of the membrane in a controlled, uniform non-equibiaxial strain state. Results The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain. Conclusion The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions.",
keywords = "Flexcell, Pressure ulcer, FEM, Shear strain",
author = "Olesen, {Christian Gammelgaard} and Pennisi, {Cristian Pablo} and {de Zee}, Mark and Vladimir Zachar and John Rasmussen",
year = "2013",
month = "5",
doi = "10.1016/j.jtv.2013.02.003",
language = "English",
volume = "22",
pages = "52--56",
journal = "Journal of Tissue Viability",
issn = "0965-206X",
publisher = "Pergamon Press",
number = "2",

}

TY - JOUR

T1 - Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures

AU - Olesen, Christian Gammelgaard

AU - Pennisi, Cristian Pablo

AU - de Zee, Mark

AU - Zachar, Vladimir

AU - Rasmussen, John

PY - 2013/5

Y1 - 2013/5

N2 - Background A modification of the Flexcell system that allows imposition of homogenous, controlled non-equibiaxial strains to cell cultures is developed and experimentally validated. The Flexcell system by default applies equibiaxial strain to cell cultures, meaning no shear strain, while soft tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis. Method The Flexcell system was redesigned using a finite element model in order to obtain large areas of the membrane in a controlled, uniform non-equibiaxial strain state. Results The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain. Conclusion The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions.

AB - Background A modification of the Flexcell system that allows imposition of homogenous, controlled non-equibiaxial strains to cell cultures is developed and experimentally validated. The Flexcell system by default applies equibiaxial strain to cell cultures, meaning no shear strain, while soft tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis. Method The Flexcell system was redesigned using a finite element model in order to obtain large areas of the membrane in a controlled, uniform non-equibiaxial strain state. Results The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain. Conclusion The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions.

KW - Flexcell

KW - Pressure ulcer

KW - FEM

KW - Shear strain

U2 - 10.1016/j.jtv.2013.02.003

DO - 10.1016/j.jtv.2013.02.003

M3 - Journal article

VL - 22

SP - 52

EP - 56

JO - Journal of Tissue Viability

JF - Journal of Tissue Viability

SN - 0965-206X

IS - 2

ER -