TY - JOUR
T1 - Enhancing Metaproteomics - The value of models and defined environmental microbial systems
AU - Herbst, Florian-Alexander
AU - Lünsmann, Vanessa
AU - Kjeldal, Henrik
AU - Jehmlich, Nico
AU - Tholey, Andreas
AU - von Bergen, Martin
AU - Nielsen, Jeppe Lund
AU - Hettich, Robert L
AU - Seifert, Jana
AU - Nielsen, Per Halkjaer
N1 - This article is protected by copyright. All rights reserved.
PY - 2016/3
Y1 - 2016/3
N2 - Metaproteomics - the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time - has provided new features to study complex microbial communities in order to unravel these "black boxes". New technical challenges arose which were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics. This article is protected by copyright. All rights reserved.
AB - Metaproteomics - the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time - has provided new features to study complex microbial communities in order to unravel these "black boxes". New technical challenges arose which were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics. This article is protected by copyright. All rights reserved.
U2 - 10.1002/pmic.201500305
DO - 10.1002/pmic.201500305
M3 - Review article
C2 - 26621789
SN - 1615-9853
VL - 16
SP - 783
EP - 798
JO - Proteomics
JF - Proteomics
IS - 5
ER -