TY - JOUR
T1 - Enrichment of activated sludge for enhanced hydrogen production from crude glycerol
AU - Varrone, Cristiano
AU - Rosa, Silvia
AU - Fiocchetti, Floriana
AU - Giussani, Barbara
AU - Izzo, Giulio
AU - Massini, Giulia
AU - Marone, Antonella
AU - Signorini, Antonella
AU - Wang, Aijie
PY - 2013/2/6
Y1 - 2013/2/6
N2 - Enriched activated sludge that can effectively convert crude glycerol into bio-hydrogen was selected by an eco-biotechnological approach, in very strict conditions, using biodiesel-derived glycerol as the only carbon source. The thus obtained functional consortium was characterized by the genera Klebsiella, Escherichia/Shigella and Cupriavidus. During enrichment, the dominant metabolic end-product shifted from a 1,3 propanediol to ethanol, with a concomitant increase of the hydrogen yield from 0.18 ± 0.003 to 0.66 ± 0.06 mol/mol and an almost five-fold increase of the hydrogen production. Glycerol degradation efficiency showed an increase of around 50%. In optimized and upscaled conditions it was possible to obtain a hydrogen production rate of 2960 mL H2/L/day ± 185 at a near stoichiometric yield (of 0.90 mol/mol ± 0.01), with a carbon recovery of almost 90%, both in sterile and non-sterile conditions. Glycerol was almost totally degraded (degradation efficiency of 97.42% ± 0.98), independently of the glycerol type used.
AB - Enriched activated sludge that can effectively convert crude glycerol into bio-hydrogen was selected by an eco-biotechnological approach, in very strict conditions, using biodiesel-derived glycerol as the only carbon source. The thus obtained functional consortium was characterized by the genera Klebsiella, Escherichia/Shigella and Cupriavidus. During enrichment, the dominant metabolic end-product shifted from a 1,3 propanediol to ethanol, with a concomitant increase of the hydrogen yield from 0.18 ± 0.003 to 0.66 ± 0.06 mol/mol and an almost five-fold increase of the hydrogen production. Glycerol degradation efficiency showed an increase of around 50%. In optimized and upscaled conditions it was possible to obtain a hydrogen production rate of 2960 mL H2/L/day ± 185 at a near stoichiometric yield (of 0.90 mol/mol ± 0.01), with a carbon recovery of almost 90%, both in sterile and non-sterile conditions. Glycerol was almost totally degraded (degradation efficiency of 97.42% ± 0.98), independently of the glycerol type used.
KW - Biodiesel
KW - Biohydrogen
KW - Crude glycerol
KW - Dark fermentation
KW - Enrichment
KW - Mixed culture
UR - http://www.scopus.com/inward/record.url?scp=84872346058&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2012.11.069
DO - 10.1016/j.ijhydene.2012.11.069
M3 - Journal article
AN - SCOPUS:84872346058
SN - 0360-3199
VL - 38
SP - 1319
EP - 1331
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 3
ER -