Epsilon haemoglobin specific antibodies with applications in noninvasive prenatal diagnosis

Morten Dræby SØrensen, Regina Gonzalez Dosal, Kim Bak Jensen, Britta Christensen, Steen KØlvraa, Uffe Birk Jensen, Peter Kristensen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

11 Citations (Scopus)

Abstract

Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising -Hb, one was chosen for further characterization, DAb1. DAb1 binds to -Hb both in Western blots and immunocytochemistry. Several -Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express -Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.

Original languageEnglish
Article number659219
JournalJournal of Biomedicine and Biotechnology
Volume2009
ISSN1110-7243
DOIs
Publication statusPublished - 24 Aug 2009
Externally publishedYes

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