Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing

Caitlin Margaret Singleton; Martin Hjorth Andersen; Rasmus Hansen Kirkegaard; Jannie Munk Kristensen; Thomas Yssing Michaelsen; Søren Michael Karst; Morten Simonsen Dueholm; Per Halkjær Nielsen; Mads Albertsen

Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing

Caitlin Margaret Singleton, Martin Hjorth Andersen, Rasmus Hansen Kirkegaard, Jannie Munk Kristensen, Thomas Yssing Michaelsen, Søren Michael Karst, Morten Simonsen Dueholm, Per Halkjær Nielsen, Mads Albertsen

Research output: Contribution to conference without publisher/journal › Poster › Research

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Abstract

Abstract: Sequencing of 23 Danish WWTP activated sludge metagenomes has produced >1 Tbp of Nanopore long-read data and 0.9 Tbp of Illumina short-read data. These data are enabling the recovery of hundreds of MAGs, including high quality and circularised complete genomes from both characterised and novel taxa, enabling us to describe the wastewater community at the genome level.

Original language	English
Publication date	18 Nov 2019
Publication status	Published - 18 Nov 2019
Event	8th IWA Microbial Ecology and Water Engineering Specialist Conference - ANA Crowne Plaza, Hiroshima, Japan Duration: 17 Nov 2019 → 20 Nov 2019 http://mewe2019.org/

Conference

Conference	8th IWA Microbial Ecology and Water Engineering Specialist Conference
Location	ANA Crowne Plaza
Country/Territory	Japan
City	Hiroshima
Period	17/11/2019 → 20/11/2019
Internet address	http://mewe2019.org/

Keywords

Nanopore
Metagenomics
Long-read
Microbial community

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Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing

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@conference{30dfc06a9f2549538ce4a2186aee468d,

title = "Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing",

abstract = "Abstract: Sequencing of 23 Danish WWTP activated sludge metagenomes has produced >1 Tbp of Nanopore long-read data and 0.9 Tbp of Illumina short-read data. These data are enabling the recovery of hundreds of MAGs, including high quality and circularised complete genomes from both characterised and novel taxa, enabling us to describe the wastewater community at the genome level. ",

keywords = "Nanopore, Metagenomics, Long-read, Microbial community",

author = "Singleton, {Caitlin Margaret} and Andersen, {Martin Hjorth} and Kirkegaard, {Rasmus Hansen} and Kristensen, {Jannie Munk} and {Yssing Michaelsen}, Thomas and Karst, {S{\o}ren Michael} and Dueholm, {Morten Simonsen} and Nielsen, {Per Halkj{\ae}r} and Mads Albertsen",

year = "2019",

month = nov,

day = "18",

language = "English",

note = "8th IWA Microbial Ecology and Water Engineering Specialist Conference ; Conference date: 17-11-2019 Through 20-11-2019",

url = "http://mewe2019.org/",

}

Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing. / Singleton, Caitlin Margaret; Andersen, Martin Hjorth; Kirkegaard, Rasmus Hansen et al.
2019. Poster presented at 8th IWA Microbial Ecology and Water Engineering Specialist Conference, Hiroshima, Japan.

Research output: Contribution to conference without publisher/journal › Poster › Research

TY - CONF

T1 - Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing

AU - Singleton, Caitlin Margaret

AU - Andersen, Martin Hjorth

AU - Kirkegaard, Rasmus Hansen

AU - Kristensen, Jannie Munk

AU - Yssing Michaelsen, Thomas

AU - Karst, Søren Michael

AU - Dueholm, Morten Simonsen

AU - Nielsen, Per Halkjær

AU - Albertsen, Mads

PY - 2019/11/18

Y1 - 2019/11/18

N2 - Abstract: Sequencing of 23 Danish WWTP activated sludge metagenomes has produced >1 Tbp of Nanopore long-read data and 0.9 Tbp of Illumina short-read data. These data are enabling the recovery of hundreds of MAGs, including high quality and circularised complete genomes from both characterised and novel taxa, enabling us to describe the wastewater community at the genome level.

AB - Abstract: Sequencing of 23 Danish WWTP activated sludge metagenomes has produced >1 Tbp of Nanopore long-read data and 0.9 Tbp of Illumina short-read data. These data are enabling the recovery of hundreds of MAGs, including high quality and circularised complete genomes from both characterised and novel taxa, enabling us to describe the wastewater community at the genome level.

KW - Nanopore

KW - Metagenomics

KW - Long-read

KW - Microbial community

M3 - Poster

T2 - 8th IWA Microbial Ecology and Water Engineering Specialist Conference

Y2 - 17 November 2019 through 20 November 2019

ER -

Extensive and High Quality Genome Recovery from full-scale WWTPs using Nanopore Sequencing

Abstract

Conference

Keywords

Access to Document

AUB Link

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