TY - JOUR
T1 - Extracellular Vesicle (EV) Array
T2 - microarray capturing of exosomes and other extracellular vesicles for multiplexed phenotyping
AU - Jørgensen, Malene
AU - Bæk, Rikke
AU - Pedersen, Shona
AU - Søndergaard, Evo Kristina Lindersson
AU - Kristensen, Søren R
AU - Varming, Kim
PY - 2013
Y1 - 2013
N2 - Exosomes are one of the several types of cell-derived vesicles with a diameter of 30-100 nm. These extracellular vesicles are recognized as potential markers of human diseases such as cancer. However, their use in diagnostic tests requires an objective and high-throughput method to define their phenotype and determine their concentration in biological fluids. To identify circulating as well as cell culture-derived vesicles, the current standard is immunoblotting or a flow cytometrical analysis for specific proteins, both of which requires large amounts of purified vesicles.
AB - Exosomes are one of the several types of cell-derived vesicles with a diameter of 30-100 nm. These extracellular vesicles are recognized as potential markers of human diseases such as cancer. However, their use in diagnostic tests requires an objective and high-throughput method to define their phenotype and determine their concentration in biological fluids. To identify circulating as well as cell culture-derived vesicles, the current standard is immunoblotting or a flow cytometrical analysis for specific proteins, both of which requires large amounts of purified vesicles.
U2 - 10.3402/jev.v2i0.20920
DO - 10.3402/jev.v2i0.20920
M3 - Journal article
C2 - 24009888
SN - 2001-3078
VL - 2
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
ER -