TY - JOUR
T1 - Food origin influences microbiota and stable isotope enrichment profiles of cold-adapted Collembola (
Desoria ruseki).
AU - Hao, Cao
AU - de Jonge, Nadieh
AU - Zhu, Dong
AU - Feng, Lichao
AU - Zhang, Bing
AU - Chen, Ting-Wen
AU - Wu, Donghui
AU - Nielsen, Jeppe Lund
N1 - Copyright © 2022 Hao, de Jonge, Zhu, Feng, Zhang, Chen, Wu and Nielsen.
PY - 2022/11/24
Y1 - 2022/11/24
N2 - Collembola are a group of globally distributed microarthropods that can tolerate low temperature and are active in extremely cold environments. While it is well known that animal diets can shape their microbiota, the microbiota of soil animals is not well described, particularly for animals with limited food resources, such as Collembola active in winter at low temperatures. In this study, we explored the effects of three different food sources; corn litter (agriculture grain residuals), Mongolian oak litter (natural plant residuals), and yeast (common food for Collembola culture), on the microbiota of a winter-active Collembola species,
Desoria ruseki. We found that microbial diversity and community composition of the Collembola were strongly altered after feeding with different food sources for 30 days. Collembola individuals fed on corn litter harbored the highest bacterial richness and were dominated by a representative of
Microbacteriaceae. In contrast, those fed on yeast exhibited the lowest bacterial richness and were primarily colonized by
Pseudomonas. The microbial communities associated with the winter-active Collembola differed significantly from those observed in the food. Collembola nutrient turnover also differed when cultured with different food sources, as indicated by the C and N stable isotopic signatures. Our study highlights microbial associations with stable isotopic enrichments of the host. Specifically, the
Arthrobacter was positively correlated with δ
13C enrichment in the host. Representatives of
Microbacteriaceae,
Micrococcaceae, TM7a,
Devosia, and
Rathayibacter were positively correlated with δ
15N enrichment of the host. Our study indicates that food sources are major determinants for Collembola microbiota that simultaneously alter consumers' isotopic niches, thereby improving our understanding of the roles played by host-microbiota interactions in sustaining soil biodiversity during the winter.
AB - Collembola are a group of globally distributed microarthropods that can tolerate low temperature and are active in extremely cold environments. While it is well known that animal diets can shape their microbiota, the microbiota of soil animals is not well described, particularly for animals with limited food resources, such as Collembola active in winter at low temperatures. In this study, we explored the effects of three different food sources; corn litter (agriculture grain residuals), Mongolian oak litter (natural plant residuals), and yeast (common food for Collembola culture), on the microbiota of a winter-active Collembola species,
Desoria ruseki. We found that microbial diversity and community composition of the Collembola were strongly altered after feeding with different food sources for 30 days. Collembola individuals fed on corn litter harbored the highest bacterial richness and were dominated by a representative of
Microbacteriaceae. In contrast, those fed on yeast exhibited the lowest bacterial richness and were primarily colonized by
Pseudomonas. The microbial communities associated with the winter-active Collembola differed significantly from those observed in the food. Collembola nutrient turnover also differed when cultured with different food sources, as indicated by the C and N stable isotopic signatures. Our study highlights microbial associations with stable isotopic enrichments of the host. Specifically, the
Arthrobacter was positively correlated with δ
13C enrichment in the host. Representatives of
Microbacteriaceae,
Micrococcaceae, TM7a,
Devosia, and
Rathayibacter were positively correlated with δ
15N enrichment of the host. Our study indicates that food sources are major determinants for Collembola microbiota that simultaneously alter consumers' isotopic niches, thereby improving our understanding of the roles played by host-microbiota interactions in sustaining soil biodiversity during the winter.
KW - feeding ecology
KW - host-microbiota interaction
KW - soil arthropods
KW - springtail
KW - stable isotope
KW - winter biodiversity
UR - http://www.scopus.com/inward/record.url?scp=85143415871&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2022.1030429
DO - 10.3389/fmicb.2022.1030429
M3 - Journal article
C2 - 36504791
SN - 1664-302X
VL - 13
SP - 1030429
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 1030429
ER -