TY - JOUR
T1 - Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi
AU - Westphal, Klaus Ringsborg
AU - Nielsen, Katrine Amalie Hamborg
AU - Wollenberg, Rasmus Dam
AU - Møllerhøj, Mathias Bonde
AU - Bachleitner, Simone
AU - Studt, Lena
AU - Lysøe, Erik
AU - Giese, Henriette
AU - Wimmer, Reinhard
AU - Sørensen, Jens Laurids
AU - Sondergaard, Teis Esben
PY - 2019/5/16
Y1 - 2019/5/16
N2 - Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.
AB - Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.
KW - ABC-transporter
KW - Fusarium
KW - Mycotoxins
KW - NRPS
KW - peptidase
KW - secondary metabolites
UR - http://www.scopus.com/inward/record.url?scp=85066282815&partnerID=8YFLogxK
U2 - 10.3390/toxins11050277
DO - 10.3390/toxins11050277
M3 - Journal article
C2 - 31100892
AN - SCOPUS:85066282815
SN - 2072-6651
VL - 11
JO - Toxins
JF - Toxins
IS - 5
M1 - 277
ER -