In this study, we aim to establish a wastewater treatment-specific reference database, comprised of full-length microbial 16S rRNA gene sequences from wastewater treatment systems across the world (the Global Database of Microbes in Wastewater Treatment Systems), by applying a novel high-throughput, primer-free sequencing method. More than 500 treatment plants from more than 35 countries are already included, making it the largest of its kind to date. The included plants cover most process designs (e.g. carbon-, nitrogen-, and phosphorus removal, and both activated sludge, granules and biofilters). Here we present an overview of procedures for sampling, sample handling, sequencing, bioinformatic pipeline, and examples of the improved reference database and taxonomy.
The prospects of the database are exciting, promising to provide a unique identification tag for all future analyses of wastewater treatment systems, and with time to link function to specific species, e.g. via updated versions of the MiDAS field guide. Furthermore, the long sequences will allow for better evaluation and design of primers for future studies.
|Translated title of the contribution||Global database over mikroorganismer i renseanlæg|
|Publication date||13 Aug 2018|
|Publication status||Published - 13 Aug 2018|
|Event||17th International Symposium on Microbial Ecology: ISME17 - Leipziger Messe Exhibition and Convention Centre, Leipzig, Germany|
Duration: 12 Aug 2018 → 17 Aug 2018
Conference number: 17
|Conference||17th International Symposium on Microbial Ecology|
|Location||Leipziger Messe Exhibition and Convention Centre|
|Period||12/08/2018 → 17/08/2018|