Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

Tobias Bruun Pedersen; Mikkel Rank Nielsen; Sebastian Birkedal Kristensen; Eva Mia Lang Spedtsberg; Wafaa Yasmine; Rikke Matthiesen; Samba Evelyne Kabemba Kaniki; Trine Sørensen; Celine Petersen; Jens Muff; Teis Esben Sondergaard; Kåre Lehmann Nielsen; Reinhard Wimmer; Jens Laurids Sørensen

doi:10.3390/ijms21207601

Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

Tobias Bruun Pedersen, Mikkel Rank Nielsen, Sebastian Birkedal Kristensen, Eva Mia Lang Spedtsberg, Wafaa Yasmine, Rikke Matthiesen, Samba Evelyne Kabemba Kaniki, Trine Sørensen, Celine Petersen, Jens Muff, Teis Esben Sondergaard, Kåre Lehmann Nielsen, Reinhard Wimmer, Jens Laurids Sørensen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

Original language	English
Article number	7601
Journal	International Journal of Molecular Sciences
Volume	21
Issue number	20
Pages (from-to)	1-10
Number of pages	10
ISSN	1661-6596
DOIs	https://doi.org/10.3390/ijms21207601
Publication status	Published - 2 Oct 2020

Keywords

Bostrycoidin
Fungi
Fusarium
Heterologous expression
Pigments
Polyketides
Yeast

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Pedersen, T. B., Nielsen, M. R., Kristensen, S. B., Spedtsberg, E. M. L., Yasmine, W., Matthiesen, R., Kaniki, S. E. K., Sørensen, T., Petersen, C., Muff, J., Sondergaard, T. E., Nielsen, K. L., Wimmer, R., & Sørensen, J. L. (2020). Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani. International Journal of Molecular Sciences , 21(20), 1-10. Article 7601. https://doi.org/10.3390/ijms21207601

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title = "Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani",

abstract = "Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.",

keywords = "Bostrycoidin, Fungi, Fusarium, Heterologous expression, Pigments, Polyketides, Yeast",

author = "Pedersen, {Tobias Bruun} and Nielsen, {Mikkel Rank} and Kristensen, {Sebastian Birkedal} and Spedtsberg, {Eva Mia Lang} and Wafaa Yasmine and Rikke Matthiesen and Kaniki, {Samba Evelyne Kabemba} and Trine S{\o}rensen and Celine Petersen and Jens Muff and Sondergaard, {Teis Esben} and Nielsen, {K{\aa}re Lehmann} and Reinhard Wimmer and S{\o}rensen, {Jens Laurids}",

note = "Funding Information: Funding: This study was supported by grants from The Danish Research Council, Technology, and Production (Grant No. 7017-00167) and the Novo Nordisk Foundation (NNF18OC0034952). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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Pedersen, TB, Nielsen, MR, Kristensen, SB, Spedtsberg, EML, Yasmine, W, Matthiesen, R, Kaniki, SEK, Sørensen, T, Petersen, C, Muff, J , Sondergaard, TE , Nielsen, KL , Wimmer, R & Sørensen, JL 2020, 'Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani', International Journal of Molecular Sciences , vol. 21, no. 20, 7601, pp. 1-10. https://doi.org/10.3390/ijms21207601

TY - JOUR

T1 - Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

AU - Pedersen, Tobias Bruun

AU - Nielsen, Mikkel Rank

AU - Kristensen, Sebastian Birkedal

AU - Spedtsberg, Eva Mia Lang

AU - Yasmine, Wafaa

AU - Matthiesen, Rikke

AU - Kaniki, Samba Evelyne Kabemba

AU - Sørensen, Trine

AU - Petersen, Celine

AU - Muff, Jens

AU - Sondergaard, Teis Esben

AU - Nielsen, Kåre Lehmann

AU - Wimmer, Reinhard

AU - Sørensen, Jens Laurids

N1 - Funding Information: Funding: This study was supported by grants from The Danish Research Council, Technology, and Production (Grant No. 7017-00167) and the Novo Nordisk Foundation (NNF18OC0034952). Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10/2

Y1 - 2020/10/2

N2 - Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

AB - Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

KW - Bostrycoidin

KW - Fungi

KW - Fusarium

KW - Heterologous expression

KW - Pigments

KW - Polyketides

KW - Yeast

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U2 - 10.3390/ijms21207601

DO - 10.3390/ijms21207601

M3 - Journal article

C2 - 33066643

AN - SCOPUS:85092926943

SN - 1661-6596

VL - 21

SP - 1

EP - 10

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 20

M1 - 7601

ER -

Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

Abstract

Keywords

UN SDGs

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