Heterologous expression of the core genes in the complex fusarubin gene cluster of Fusarium Solani

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Abstract

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

Original languageEnglish
Article number7601
JournalInternational Journal of Molecular Sciences
Volume21
Issue number20
Pages (from-to)1-10
Number of pages10
ISSN1661-6596
DOIs
Publication statusPublished - 2 Oct 2020

Keywords

  • Bostrycoidin
  • Fungi
  • Fusarium
  • Heterologous expression
  • Pigments
  • Polyketides
  • Yeast

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