Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

Jens Laurids Sørensen; Teis Esben Sondergaard; Lorenzo Covarelli; Patricia Romans Fuertes; Frederik Teilfeldt Hansen; Rasmus John Normand Frandsen; Wagma Saei; Mie Bech Lukassen; Reinhard Wimmer; Kristian Fog Nielsen; Donald Max Gardiner; Henriette Giese

doi:10.1021/np500436r

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

Jens Laurids Sørensen, Teis Esben Sondergaard, Lorenzo Covarelli, Patricia Romans Fuertes, Frederik Teilfeldt Hansen, Rasmus John Normand Frandsen, Wagma Saei, Mie Bech Lukassen, Reinhard Wimmer, Kristian Fog Nielsen, Donald Max Gardiner, Henriette Giese

Research output: Contribution to journal › Journal article › Research › peer-review

49 Citations (Scopus)

Abstract

The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

Original language	English
Journal	Journal of Natural Products
Volume	77
Issue number	12
Pages (from-to)	2619-2625
Number of pages	7
ISSN	0163-3864
DOIs	https://doi.org/10.1021/np500436r
Publication status	Published - 26 Dec 2014

Bibliographical note

Publisher Copyright:
© 2014 The American Chemical Society and American Society of Pharmacognosy.

Access to Document

10.1021/np500436r

AUB Link

Search for the material in Aalborg University Library's search engine

Cite this

Sørensen, J. L., Sondergaard, T. E., Covarelli, L., Fuertes, P. R., Hansen, F. T., Frandsen, R. J. N., Saei, W., Lukassen, M. B., Wimmer, R., Nielsen, K. F., Gardiner, D. M., & Giese, H. (2014). Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum. Journal of Natural Products, 77(12), 2619-2625. https://doi.org/10.1021/np500436r

@article{28fc46ec05c94b8e8d10b27b8aca7cfe,

title = "Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum",

abstract = "The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.",

author = "S{\o}rensen, {Jens Laurids} and Sondergaard, {Teis Esben} and Lorenzo Covarelli and Fuertes, {Patricia Romans} and Hansen, {Frederik Teilfeldt} and Frandsen, {Rasmus John Normand} and Wagma Saei and Lukassen, {Mie Bech} and Reinhard Wimmer and Nielsen, {Kristian Fog} and Gardiner, {Donald Max} and Henriette Giese",

note = "Publisher Copyright: {\textcopyright} 2014 The American Chemical Society and American Society of Pharmacognosy.",

year = "2014",

month = dec,

day = "26",

doi = "10.1021/np500436r",

language = "English",

volume = "77",

pages = "2619--2625",

journal = "Journal of Natural Products",

issn = "0163-3864",

publisher = "American Chemical Society",

number = "12",

}

Sørensen, JL , Sondergaard, TE, Covarelli, L, Fuertes, PR, Hansen, FT, Frandsen, RJN, Saei, W , Lukassen, MB , Wimmer, R, Nielsen, KF, Gardiner, DM & Giese, H 2014, 'Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum', Journal of Natural Products, vol. 77, no. 12, pp. 2619-2625. https://doi.org/10.1021/np500436r

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum. / Sørensen, Jens Laurids ; Sondergaard, Teis Esben; Covarelli, Lorenzo et al.
In: Journal of Natural Products, Vol. 77, No. 12, 26.12.2014, p. 2619-2625.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

AU - Sørensen, Jens Laurids

AU - Sondergaard, Teis Esben

AU - Covarelli, Lorenzo

AU - Fuertes, Patricia Romans

AU - Hansen, Frederik Teilfeldt

AU - Frandsen, Rasmus John Normand

AU - Saei, Wagma

AU - Lukassen, Mie Bech

AU - Wimmer, Reinhard

AU - Nielsen, Kristian Fog

AU - Gardiner, Donald Max

AU - Giese, Henriette

PY - 2014/12/26

Y1 - 2014/12/26

N2 - The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

AB - The closely related species Fusarium graminearum and Fusarium pseudograminearum differ in that each contains a gene cluster with a polyketide synthase (PKS) and a nonribosomal peptide synthetase (NRPS) that is not present in the other species. To identify their products, we deleted PKS6 and NRPS7 in F. graminearum and NRPS32 in F. pseudograminearum. By comparing the secondary metabolite profiles of the strains we identified the resulting product in F. graminearum as fusaristatin A, and as W493 A and B in F. pseudograminearum. These lipopeptides have previously been isolated from unidentified Fusarium species. On the basis of genes in the putative gene clusters we propose a model for biosynthesis where the polyketide product is shuttled to the NPRS via a CoA ligase and a thioesterase in F. pseudograminearum. In F. graminearum the polyketide is proposed to be directly assimilated by the NRPS.

UR - http://www.scopus.com/inward/record.url?scp=84920165664&partnerID=8YFLogxK

U2 - 10.1021/np500436r

DO - 10.1021/np500436r

M3 - Journal article

C2 - 25412204

SN - 0163-3864

VL - 77

SP - 2619

EP - 2625

JO - Journal of Natural Products

JF - Journal of Natural Products

IS - 12

ER -

Identification of the Biosynthetic Gene Clusters for the Lipopeptides Fusaristatin A and W493 B in Fusarium graminearum and F. pseudograminearum

Abstract

Bibliographical note

Access to Document

AUB Link

Other files and links

Fingerprint

Cite this