Abstract
SNAP-23 has an important role in protein-trafficking processes in mammalian cells and until yet two isoforms of SNAP-23 (SNAP-23a and SNAP-23b) have been described. In the present report, we have identified the existence of three new SNAP-23 isoforms (named SNAP-23c, SNAP-23d, and SNAP-23e), which arise from alternative splicing. By RT-PCR all five splice variants were shown to be expressed in four different human inflammatory cells (eosinophils, basophils, neutrophils, and peripheral blood mononuclear cells). Transfection of the human basophilic KU-812 cell line with plasmid constructs containing the cDNAs of the five splice variants located SNAP-23a and SNAP-23b primarily in the plasma membrane. The other three splice variants were localized both intracellularly and in the plasma membrane.
| Original language | English |
|---|---|
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 285 |
| Issue number | 2 |
| Pages (from-to) | 320-7 |
| Number of pages | 8 |
| ISSN | 0006-291X |
| DOIs | |
| Publication status | Published - 13 Jul 2001 |
| Externally published | Yes |
Keywords
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Basophils
- Carrier Proteins
- Cell Line
- Eosinophils
- Exons
- Genetic Variation
- Granulocytes
- Humans
- Introns
- Leukocytes, Mononuclear
- Mammals
- Molecular Sequence Data
- Neutrophils
- Protein Isoforms
- Qb-SNARE Proteins
- Qc-SNARE Proteins
- Recombinant Fusion Proteins
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Amino Acid
- Transcription, Genetic
- Transfection