Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns

Steffen B. Petersen, Ane Kold Di Gennaro, Teresa Neves Petersen, Esben Skovsen, Antonietta Parracino

Research output: Contribution to journalJournal articleResearchpeer-review

9 Citations (Scopus)

Abstract

We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 mu m) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed. (C) 2010 Optical Society of America
Original languageEnglish
JournalApplied Optics
Volume49
Issue number28
Pages (from-to)5344-5350
ISSN0003-6935
DOIs
Publication statusPublished - 2010

Keywords

  • Fourier transform
  • diffraction pattern
  • UV light assisted molecular immobilisation
  • high density biosensors
  • arrays

Cite this

Petersen, Steffen B. ; Gennaro, Ane Kold Di ; Neves Petersen, Teresa ; Skovsen, Esben ; Parracino, Antonietta. / Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns. In: Applied Optics. 2010 ; Vol. 49, No. 28. pp. 5344-5350.
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Immobilization of biomolecules onto surfaces according to ultraviolet light diffraction patterns. / Petersen, Steffen B.; Gennaro, Ane Kold Di; Neves Petersen, Teresa; Skovsen, Esben; Parracino, Antonietta.

In: Applied Optics, Vol. 49, No. 28, 2010, p. 5344-5350.

Research output: Contribution to journalJournal articleResearchpeer-review

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AU - Petersen, Steffen B.

AU - Gennaro, Ane Kold Di

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AB - We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstrate that, by shaping the pattern of the UV light used to induce molecular immobilization, one can control the pattern of immobilized molecules onto the surface. Using a single-aperture spatial mask, combined with the Fourier transforming property of a focusing lens, we show that submicrometer (0.7 mu m) resolved patterns of immobilized prostate-specific antigen biomolecules can be created. If a dual-aperture spatial mask is used, the results differ from the expected Fourier transform pattern of the mask. It appears as a superposition of two diffraction patterns produced by the two apertures, with a fine structured interference pattern superimposed. (C) 2010 Optical Society of America

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