Impact of storage conditions on the methanogenic activity of anaerobic digestion inocula

Sergi Astals; Konrad Koch; Sören Weinrich; Sasha D. Hafner; Stephan Tait; Miriam Peces

doi:10.3390/W12051321

Impact of storage conditions on the methanogenic activity of anaerobic digestion inocula

Sergi Astals^*, Konrad Koch, Sören Weinrich, Sasha D. Hafner, Stephan Tait, Miriam Peces

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The impact of storage temperature (4, 22 and 37 °C) and storage time (7, 14 and 21 days) on anaerobic digestion inocula was investigated through specific methanogenic activity assays. Experimental results showed that methanogenic activity decreased over time with storage, regardless of storage temperature. However, the rate at which the methanogenic activity decreased was two and five times slower at 4 °C than at 22 and 37 °C, respectively. The inoculum stored at 4 °C and room temperature (22 °C) maintained methanogenic activity close to that of fresh inoculum for 14 days (<10% difference). However, a storage temperature of 4 °C is preferred because of the slower decrease in activity with lengthier storage time. From this research, it was concluded that inoculum storage time should generally be kept to a minimum, but that storage at 4 °C could help maintain methanogenic activity for longer.

Original language	English
Article number	1321
Journal	Water (Switzerland)
Volume	12
Issue number	5
ISSN	2073-4441
DOIs	https://doi.org/10.3390/W12051321
Publication status	Published - 1 May 2020

Bibliographical note

Funding Information:
Funding: Sergi Astals is grateful to the Spanish Ministry of Science, Innovation and Universities for his Ramon y Cajal fellowship (RYC-2017-22372) and the Australian Research Council for his DECRA fellowship (DE170100497).

Publisher Copyright:
© 2020 by the authors.

Keywords

Anaerobic digestion
Biochemical methane potential (BMP) test
Biogas
Inoculum
Methanogenesis
Sample storage
Specific methanogenic activity (SMA)

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title = "Impact of storage conditions on the methanogenic activity of anaerobic digestion inocula",

abstract = "The impact of storage temperature (4, 22 and 37 °C) and storage time (7, 14 and 21 days) on anaerobic digestion inocula was investigated through specific methanogenic activity assays. Experimental results showed that methanogenic activity decreased over time with storage, regardless of storage temperature. However, the rate at which the methanogenic activity decreased was two and five times slower at 4 °C than at 22 and 37 °C, respectively. The inoculum stored at 4 °C and room temperature (22 °C) maintained methanogenic activity close to that of fresh inoculum for 14 days (<10% difference). However, a storage temperature of 4 °C is preferred because of the slower decrease in activity with lengthier storage time. From this research, it was concluded that inoculum storage time should generally be kept to a minimum, but that storage at 4 °C could help maintain methanogenic activity for longer.",

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note = "Funding Information: Funding: Sergi Astals is grateful to the Spanish Ministry of Science, Innovation and Universities for his Ramon y Cajal fellowship (RYC-2017-22372) and the Australian Research Council for his DECRA fellowship (DE170100497). Publisher Copyright: {\textcopyright} 2020 by the authors.",

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AU - Koch, Konrad

AU - Weinrich, Sören

AU - Hafner, Sasha D.

AU - Tait, Stephan

AU - Peces, Miriam

N1 - Funding Information: Funding: Sergi Astals is grateful to the Spanish Ministry of Science, Innovation and Universities for his Ramon y Cajal fellowship (RYC-2017-22372) and the Australian Research Council for his DECRA fellowship (DE170100497). Publisher Copyright: © 2020 by the authors.

PY - 2020/5/1

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N2 - The impact of storage temperature (4, 22 and 37 °C) and storage time (7, 14 and 21 days) on anaerobic digestion inocula was investigated through specific methanogenic activity assays. Experimental results showed that methanogenic activity decreased over time with storage, regardless of storage temperature. However, the rate at which the methanogenic activity decreased was two and five times slower at 4 °C than at 22 and 37 °C, respectively. The inoculum stored at 4 °C and room temperature (22 °C) maintained methanogenic activity close to that of fresh inoculum for 14 days (<10% difference). However, a storage temperature of 4 °C is preferred because of the slower decrease in activity with lengthier storage time. From this research, it was concluded that inoculum storage time should generally be kept to a minimum, but that storage at 4 °C could help maintain methanogenic activity for longer.

AB - The impact of storage temperature (4, 22 and 37 °C) and storage time (7, 14 and 21 days) on anaerobic digestion inocula was investigated through specific methanogenic activity assays. Experimental results showed that methanogenic activity decreased over time with storage, regardless of storage temperature. However, the rate at which the methanogenic activity decreased was two and five times slower at 4 °C than at 22 and 37 °C, respectively. The inoculum stored at 4 °C and room temperature (22 °C) maintained methanogenic activity close to that of fresh inoculum for 14 days (<10% difference). However, a storage temperature of 4 °C is preferred because of the slower decrease in activity with lengthier storage time. From this research, it was concluded that inoculum storage time should generally be kept to a minimum, but that storage at 4 °C could help maintain methanogenic activity for longer.

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KW - Biochemical methane potential (BMP) test

KW - Biogas

KW - Inoculum

KW - Methanogenesis

KW - Sample storage

KW - Specific methanogenic activity (SMA)

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JF - Water (Switzerland)

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Impact of storage conditions on the methanogenic activity of anaerobic digestion inocula

Abstract

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