In vitro models for ASD-patient-derived iPSCs and cerebral organoids

Sonja Simone Hohmann; Mirolyuba Ilieva; Tanja Maria Michel

doi:10.1016/bs.pmbts.2020.04.019

In vitro models for ASD-patient-derived iPSCs and cerebral organoids

Sonja Simone Hohmann^*, Mirolyuba Ilieva, Tanja Maria Michel

^*Corresponding author for this work

Research output: Contribution to book/anthology/report/conference proceeding › Book chapter › Research › peer-review

3 Citations (Scopus)

Abstract

Autism spectrum disorder (ASD) is a set of pervasive neurodevelopmental disorders. The causation is multigenic in most cases, which makes it difficult to model the condition in vitro. Advances in pluripotent stem cell technology has made it possible to generate in vitro models of human brain development. Induced pluripotent stem cells (iPSCs) can be generated from somatic cells and have the ability to differentiate to all of the body's cells. This chapter aims to give an overview of the iPSC technology for generating neural cells and cerebral organoids as models for neurodevelopment and how these models are utilized in the study of ASD. The combination of iPSC technology and the genetic modification tool CRISPR/Cas9 is described, and current limitations and future perspectives of iPSC technology is discussed.

Original language	English
Title of host publication	Autism
Editors	Mirolyuba Ilieva, Way Kwok-Wai Lau
Number of pages	21
Volume	173
Publisher	Elsevier Science Publishers B.V.
Publication date	2020
Pages	355-375
Chapter	13
ISBN (Print)	9780128212424
DOIs	https://doi.org/10.1016/bs.pmbts.2020.04.019
Publication status	Published - 2020
Externally published	Yes

Series	Progress in Molecular Biology and Translational Science
Volume	173
ISSN	1877-1173

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc.

Keywords

Autism spectrum disorder (ASD)
Brain organoids
Cerebral organoids
CRISPR/Cas9
Differentiation
Induced pluripotent stem cells (iPSC)
Neurodevelopment modeling

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10.1016/bs.pmbts.2020.04.019

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abstract = "Autism spectrum disorder (ASD) is a set of pervasive neurodevelopmental disorders. The causation is multigenic in most cases, which makes it difficult to model the condition in vitro. Advances in pluripotent stem cell technology has made it possible to generate in vitro models of human brain development. Induced pluripotent stem cells (iPSCs) can be generated from somatic cells and have the ability to differentiate to all of the body's cells. This chapter aims to give an overview of the iPSC technology for generating neural cells and cerebral organoids as models for neurodevelopment and how these models are utilized in the study of ASD. The combination of iPSC technology and the genetic modification tool CRISPR/Cas9 is described, and current limitations and future perspectives of iPSC technology is discussed.",

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volume = "173",

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In vitro models for ASD-patient-derived iPSCs and cerebral organoids. / Hohmann, Sonja Simone; Ilieva, Mirolyuba; Michel, Tanja Maria.
Autism. ed. / Mirolyuba Ilieva; Way Kwok-Wai Lau. Vol. 173 Elsevier Science Publishers B.V., 2020. p. 355-375 (Progress in Molecular Biology and Translational Science, Vol. 173).

Research output: Contribution to book/anthology/report/conference proceeding › Book chapter › Research › peer-review

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AU - Michel, Tanja Maria

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In vitro models for ASD-patient-derived iPSCs and cerebral organoids

Abstract

Bibliographical note

Keywords

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