Major Proteomic Changes Associated with Amyloid-Induced Biofilm Formation in Pseudomonas aeruginosa PAO1

Florian-Alexander Herbst, Mads Toft Søndergaard, Henrik Kjeldal, Allan Stensballe, Per Halkjær Nielsen, Morten Simonsen Dueholm

Research output: Contribution to journalJournal articleResearchpeer-review

31 Citations (Scopus)

Abstract

The newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa. However, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap-mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloids were found to not solely act as inert structural biofilm components. Their presence induced major changes in the global proteome of the bacterium. These included the lowered abundance of classical virulence factors such as elastase B and the secretion system of alkaline protease A. Amyloid-mediated biofilm formation furthermore increased abundance of the alginate and pyoverdine synthesis machinery, turning P. aeruginosa PAO1 into an unexpected mucoid phenotype. The results imply a significant impact of functional amyloids on the physiology of P. aeruginosa with subsequent implications for biofilm formation and chronic infections.

Original languageEnglish
JournalJournal of Proteome Research
Volume14
Issue number1
Pages (from-to)72-81
Number of pages10
ISSN1535-3893
DOIs
Publication statusPublished - 2015

Fingerprint

Dive into the research topics of 'Major Proteomic Changes Associated with Amyloid-Induced Biofilm Formation in Pseudomonas aeruginosa PAO1'. Together they form a unique fingerprint.

Cite this