Abstract
The usage of Xenon (Xe) is known in anesthesia and biobanking areas. It is considered preservation effect of Xe is associated either with clathrate formation - solid gaseous structures or dissolution of Xe molecules in liquid phase without physical state modification (so-called hyperbarium) [1]. This study is addressed to establish differences between hyberbarium or clathrate Xe actions as well as its applications on various bioobjects with anaerobic - red blood cells (RBCs) and aerobic (skin fragments) metabolism. Xe clathrates and hyperbarium storage were simulated under 277 K and 620-725 kPa and 300-350 kPa, respectively. RBCs were divided at three groups: clathrates, hyperbarium and CPDA-1 as control and stored for 30 days. It was demonstrated that the hemolysis index in clathrate group (29.65 ± 3.71, CI95%) was significantly higher than in hyberbarium (17.81 ± 2.75, CI95%) as compared to control (15.68 ± 1.11, CI95%). Skin fragments were harvested from rat tails and divided on hyberbarium, clathrate and dimetylsulfoxide cryopreserved as control group and stored for 7 days. Assessment was performed by point-score method including epidermal-dermal integrity various assays and engraftment index. It was shown that scores in clathrate group (7.7 ± 0.7, CI95%) was significantly higher than in hyperbarium (5.3 ± 2.2, CI95%) and control (6.0 ± 1.7, CI95%). The results highlighted the differences between anaerobic and aerobic metabolism. The advantages of either gaseous (hyperbarium) or solid Xe state (clathrates) for tissue and RBCs have been clarified.
Original language | English |
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Article number | 161 |
Journal | Tissue Engineering. Part A |
Volume | 23 |
Issue number | S1 |
Pages (from-to) | S43-S43 |
Number of pages | 1 |
ISSN | 1937-3341 |
DOIs | |
Publication status | Published - 2017 |
Event | 2017 TERMIS - Americas Conference & Exhibition - Charlotte, NC, Charlotte, NC, United States Duration: 3 Dec 2017 → 6 Dec 2017 |
Conference
Conference | 2017 TERMIS - Americas Conference & Exhibition |
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Location | Charlotte, NC |
Country/Territory | United States |
City | Charlotte, NC |
Period | 03/12/2017 → 06/12/2017 |