The usage of Xenon (Xe) is known in anesthesia and biobanking areas. It is considered preservation effect of Xe is associated either with clathrate formation - solid gaseous structures or dissolution of Xe molecules in liquid phase without physical state modification (so-called hyperbarium) . This study is addressed to establish differences between hyberbarium or clathrate Xe actions as well as its applications on various bioobjects with anaerobic - red blood cells (RBCs) and aerobic (skin fragments) metabolism. Xe clathrates and hyperbarium storage were simulated under 277 K and 620-725 kPa and 300-350 kPa, respectively. RBCs were divided at three groups: clathrates, hyperbarium and CPDA-1 as control and stored for 30 days. It was demonstrated that the hemolysis index in clathrate group (29.65 ± 3.71, CI95%) was significantly higher than in hyberbarium (17.81 ± 2.75, CI95%) as compared to control (15.68 ± 1.11, CI95%). Skin fragments were harvested from rat tails and divided on hyberbarium, clathrate and dimetylsulfoxide cryopreserved as control group and stored for 7 days. Assessment was performed by point-score method including epidermal-dermal integrity various assays and engraftment index. It was shown that scores in clathrate group (7.7 ± 0.7, CI95%) was significantly higher than in hyperbarium (5.3 ± 2.2, CI95%) and control (6.0 ± 1.7, CI95%). The results highlighted the differences between anaerobic and aerobic metabolism. The advantages of either gaseous (hyperbarium) or solid Xe state (clathrates) for tissue and RBCs have been clarified.
|Journal||Tissue Engineering. Part A|
|Number of pages||1|
|Publication status||Published - 2017|
|Event||2017 TERMIS - Americas Conference & Exhibition - Charlotte, NC, Charlotte, NC, United States|
Duration: 3 Dec 2017 → 6 Dec 2017
|Conference||2017 TERMIS - Americas Conference & Exhibition|
|Period||03/12/2017 → 06/12/2017|