Mechanisms of Xenon Effect on Skin and Red Blood Cells

Alexander Ponomarev, V. Rodin, Leonid Gurevich, Oleg Makeev

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Abstract

The usage of Xenon (Xe) is known in anesthesia and biobanking areas. It is considered preservation effect of Xe is associated either with clathrate formation - solid gaseous structures or dissolution of Xe molecules in liquid phase without physical state modification (so-called hyperbarium) [1]. This study is addressed to establish differences between hyberbarium or clathrate Xe actions as well as its applications on various bioobjects with anaerobic - red blood cells (RBCs) and aerobic (skin fragments) metabolism. Xe clathrates and hyperbarium storage were simulated under 277 K and 620-725 kPa and 300-350 kPa, respectively. RBCs were divided at three groups: clathrates, hyperbarium and CPDA-1 as control and stored for 30 days. It was demonstrated that the hemolysis index in clathrate group (29.65 ± 3.71, CI95%) was significantly higher than in hyberbarium (17.81 ± 2.75, CI95%) as compared to control (15.68 ± 1.11, CI95%). Skin fragments were harvested from rat tails and divided on hyberbarium, clathrate and dimetylsulfoxide cryopreserved as control group and stored for 7 days. Assessment was performed by point-score method including epidermal-dermal integrity various assays and engraftment index. It was shown that scores in clathrate group (7.7 ± 0.7, CI95%) was significantly higher than in hyperbarium (5.3 ± 2.2, CI95%) and control (6.0 ± 1.7, CI95%). The results highlighted the differences between anaerobic and aerobic metabolism. The advantages of either gaseous (hyperbarium) or solid Xe state (clathrates) for tissue and RBCs have been clarified.
Original languageEnglish
Article number161
JournalTissue Engineering. Part A
Volume23
Issue numberS1
Pages (from-to)S43-S43
Number of pages1
ISSN1937-3341
DOIs
Publication statusPublished - 2017
Event2017 TERMIS - Americas Conference & Exhibition - Charlotte, NC, Charlotte, NC, United States
Duration: 3 Dec 20176 Dec 2017

Conference

Conference2017 TERMIS - Americas Conference & Exhibition
LocationCharlotte, NC
CountryUnited States
CityCharlotte, NC
Period03/12/201706/12/2017

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