Abstract
The invention provides in a first aspect a method for producing an antifungal peptide in a filamentous fungal host cell, comprising expressing the antifungal peptide in the host cell, wherein an endogenous glucosyl ceramide synthase activity (EC 2.4.1.80) in the filamentous fungal host has been completely or partially inactivated compared to a parent filamentous fungal host grown under identical conditions.
In a second aspect the invention relates to a method for producing an antifungal peptide in a filamentous fungal host cell, comprising expressing the antifungal peptide in the host cell, wherein an endogenous activity encoded by: (a) the nucleotide sequence shown in SEQ ID NO: 1 , or (b) a nucleotide sequence having at least 45% identity to SEQ ID NO: 1 , or (c) a nucleotide sequence which hybridizes under at least medium stringency conditions with SEQ ID NO: 1 , or (d) a nucleotide sequence encoding a polypeptide having an amino acid sequence which has at least 50% identity with the amino acid sequence of SEQ ID NO: 2, is eliminated or reduced with more than about 60%, preferably more than about 85%, more preferably more than about 90%, and most preferably more than about 95% compared to a parent filamentous fungal host grown under identical conditions.
In a third aspect the invention relates to an Aspergillus host cell, wherein an endogenous activity has been completely or partially inactivated, and wherein the endogenous activity is encoded by: (a) the nucleotide sequence shown in SEQ ID NO: 1 , or (b) a nucleotide sequence having at least 60% identity to SEQ ID NO: 1 , or (c) a nucleotide sequence which hybridizes under at least medium stringency conditions with SEQ ID NO: 1 , or (d) a nucleotide sequence encoding a polypeptide having an amino acid sequence which has at least 70% identity with the amino acid sequence of SEQ ID NO: 2.
In a second aspect the invention relates to a method for producing an antifungal peptide in a filamentous fungal host cell, comprising expressing the antifungal peptide in the host cell, wherein an endogenous activity encoded by: (a) the nucleotide sequence shown in SEQ ID NO: 1 , or (b) a nucleotide sequence having at least 45% identity to SEQ ID NO: 1 , or (c) a nucleotide sequence which hybridizes under at least medium stringency conditions with SEQ ID NO: 1 , or (d) a nucleotide sequence encoding a polypeptide having an amino acid sequence which has at least 50% identity with the amino acid sequence of SEQ ID NO: 2, is eliminated or reduced with more than about 60%, preferably more than about 85%, more preferably more than about 90%, and most preferably more than about 95% compared to a parent filamentous fungal host grown under identical conditions.
In a third aspect the invention relates to an Aspergillus host cell, wherein an endogenous activity has been completely or partially inactivated, and wherein the endogenous activity is encoded by: (a) the nucleotide sequence shown in SEQ ID NO: 1 , or (b) a nucleotide sequence having at least 60% identity to SEQ ID NO: 1 , or (c) a nucleotide sequence which hybridizes under at least medium stringency conditions with SEQ ID NO: 1 , or (d) a nucleotide sequence encoding a polypeptide having an amino acid sequence which has at least 70% identity with the amino acid sequence of SEQ ID NO: 2.
| Translated title of the contribution | METHODE TIL AT PRODUCERE ET ANTIFUNGALT PEPTID I EN FILAMENTIØS SVAMPE VÆRTCELLE |
|---|---|
| Original language | English |
| Patent number | WO/2008/101847 |
| Filing date | 13/02/2008 |
| Country/Territory | Denmark |
| Publication status | Published - 28 Aug 2008 |
| Externally published | Yes |