MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Morten Kam Dahl Dueholm*, Kasper Skytte Andersen, Anne Kirstine C. Korntved, Vibeke Rudkjøbing, Madalena Alves, Yadira Bajón-Fernández, Damien Batstone, Caitlyn Butler, Mercedes Cecilia Cruz, Åsa Davidsson, Leonardo Erijman, Christof Holliger, Konrad Koch, Norbert Kreuzinger, Changsoo Lee, Gerasimos Lyberatos, Srikanth Mutnuri, Vincent O’Flaherty, Piotr Oleskowicz-Popiel, Dana PokornaVeronica Rajal, Michael Recktenwald, Jorge Rodríguez, Pascal E. Saikaly, Nick Tooker, Julia Vierheilig, Jo De Vrieze, Christian Wurzbacher, Per Halkjær Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

4 Citations (Scopus)

Abstract

Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 84–99% and 18–61% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process.

Original languageEnglish
Article number5361
JournalNature Communications
Volume15
ISSN2041-1723
DOIs
Publication statusPublished - 25 Jun 2024

Bibliographical note

© 2024. The Author(s).

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