Projects per year
Abstract
Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 84–99% and 18–61% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process.
Original language | English |
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Article number | 5361 |
Journal | Nature Communications |
Volume | 15 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 25 Jun 2024 |
Bibliographical note
© 2024. The Author(s).Fingerprint
Dive into the research topics of 'MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters'. Together they form a unique fingerprint.Projects
- 2 Finished
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Illumination of microbial dark matter
Nielsen, P. H. (PI), Dueholm, M. K. D. (Project Participant), Nierychlo, M. (Project Participant), Albertsen, M. (Project Participant), Hove Hansen, S. (Other), Dottorini, G. (Project Participant), Singleton, C. M. (Project Participant), Kondrotaite, Z. (Project Participant), Petersen, J. F. (Project Participant), Petriglieri, F. (PI) & Peces Gomez, M. (Project Participant)
01/01/2017 → 31/12/2024
Project: Research
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Unbiased characterization of microbial communities
Nielsen, P. H. (PI), Albertsen, M. (Project Participant) & Kristensen, J. M. (Project Participant)
01/01/2016 → 31/12/2019
Project: Research