MiDAS Field Guide – a Comprehensive Online Source of Information About the Microbes of Activated Sludge

Marta Nierychlo, Simon Jon McIlroy, Aaron Marc Saunders, Mads Albertsen, Aviaja Anna Hansen, Søren Michael Karst, Jeppe Lund Nielsen, Per Halkjær Nielsen

Research output: Contribution to conference without publisher/journalPosterResearch


activated sludge wastewater treatment systems, linking their identity with available information on their function and distribution. The guide includes
the approx. 100 abundant genera that are present in most treatment plants (based on a survey of 25 full-scale Danish wastewater treatment plants
over 6 years) along with selected bacteria with known associations with nutrient turnover and operational problems.
In addition, the SILVA taxonomy has been manually curated with annotations for all the abundant and important genera in activated sludge, many
of which are known only from sequence data that would be otherwise excluded from analyses. The MiDAS database importantly provides a common
taxonomy for the field that gives a solid foundation for the study of microbial ecology of the activated sludge process and related wastewater
treatment processes, such as biofilms and granular sludge.
Each genus in MiDAS collection has an up-to-date description of all the important metabolic features, morphology, available FISH probes, genomic
data and other relevant information based on the published literature.
Additionally, the protocols for DNA extraction and 16S Amplicon Sequencing sample preparation are provided.
The MiDAS Field Guide is a continuously developing resource where all working in the field are invited to contribute.
Original languageEnglish
Publication date18 May 2015
Publication statusPublished - 18 May 2015
EventIWA Specialist Conference: Nutrient Removal and Recovery: moving innovation into practice - Gdansk, Poland
Duration: 18 May 201521 May 2015


ConferenceIWA Specialist Conference


  • activated sludge, nutrient removal, taxonomic assignment


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