TY - JOUR
T1 - miR-21 is up-regulated in psoriasis and suppresses T cell apoptosis
AU - Meisgen, F.
AU - Xu, Ning
AU - Wei, T.
AU - Janson, P.C.
AU - Obad, S.
AU - Broom, O.
AU - Nagy, N.
AU - Kauppinen, Sakari
AU - Kemény, L.
AU - Ståhle, M.
AU - Pivarcsi, A.
AU - Sonkoly, E.
PY - 2012/4/1
Y1 - 2012/4/1
N2 - MicroRNAs are short non-coding RNAs that regulate gene expression. Previously, in a genome-wide screen, we found deregulation of microRNA expression in psoriasis skin. MicroRNA-21 (miR-21) is one of the microRNAs significantly up-regulated in psoriasis skin lesions. To identify the cell type responsible for the increased miR-21 level, we compared expression of miR-21 in epidermal cells and dermal T cells between psoriasis and healthy skin and found elevated levels of miR-21 in psoriasis in both cell types. In cultured T cells, expression of miR-21 increased markedly upon activation. To explore the function of miR-21 in primary human T helper cells, we inhibited miR-21 using a tiny seed-targeting LNA-anti-miR. Specific inhibition of miR-21 increased the apoptosis rate of activated T cells. Our results suggest that miR-21 suppresses apoptosis in activated T cells, and thus, overexpression of miR-21 may contribute to T cell-derived psoriatic skin inflammation.
AB - MicroRNAs are short non-coding RNAs that regulate gene expression. Previously, in a genome-wide screen, we found deregulation of microRNA expression in psoriasis skin. MicroRNA-21 (miR-21) is one of the microRNAs significantly up-regulated in psoriasis skin lesions. To identify the cell type responsible for the increased miR-21 level, we compared expression of miR-21 in epidermal cells and dermal T cells between psoriasis and healthy skin and found elevated levels of miR-21 in psoriasis in both cell types. In cultured T cells, expression of miR-21 increased markedly upon activation. To explore the function of miR-21 in primary human T helper cells, we inhibited miR-21 using a tiny seed-targeting LNA-anti-miR. Specific inhibition of miR-21 increased the apoptosis rate of activated T cells. Our results suggest that miR-21 suppresses apoptosis in activated T cells, and thus, overexpression of miR-21 may contribute to T cell-derived psoriatic skin inflammation.
UR - http://www.scopus.com/inward/record.url?scp=84858397752&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0625.2012.01462.x
DO - 10.1111/j.1600-0625.2012.01462.x
M3 - Journal article
AN - SCOPUS:84858397752
SN - 1600-0625
VL - 21
SP - 312
EP - 314
JO - Experimental Dermatology Online
JF - Experimental Dermatology Online
IS - 4
ER -