The two new esterases, EP6 and EP10, from Pseudomonas marginata were studied with respect to their substrate specificities and selectivities. Esters of aliphatic and cyclic secondary alcohols were shown to be substrates for EP10, which could be accepted stereoselectively in hydrolysis and transesterification reactions. EP6 showed no selectivity in hydrolysis or the studied substrates.
- Pseudomonas marginata
- Secondary alcohols