Novel mAbs reveal potent co-stimulatory activity of murine CD27

Loes A. Gravestein, John D. Nieland, Ada M. Kruisbeek, Jannie Borst*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

82 Citations (Scopus)

Abstract

Members of the tumor necrosis factor receptor (TNFR) family are emerging as important molecules implicated in the regulation of proliferation, differentiation and survival of T and B lymphocytes. Among these receptors is CD27, the function of which has thus far only been studied in the human system, where it amplifies the T cell proliferative response induced by TCR triggering. We report here the generation of mAbs to murine CD27, by an efficient method involving the use of transfected Armenian hamster fibroblasts. Previous analysis had already indicated that murine CD27 mRNA is uniquely expressed in lymphold cells. As determined with one of the newly developed antibodies, murine CD27 is expressed on the great majority of both αβ and γδ T lymphocytes, on a small population of peripheral B cells, and on a very small subset of B220+ cells in the bone marrow. This distribution largely corresponds to that in the human system. However, unlike human CD27, which is primarily expressed in mature, medullary thymocytes, murine CD27 is found on all thymocytes, except a subset of CD4-CD8- precursors. Upon cross-linking, anti-CD27 mAb amplified the proliferative response of purified T lymphocytes to suboptimal stimulation with concanavalin A at least 4-fold. This indicates that such mAbs can mimick llgand binding and demonstrates that CD27 also acts as a potent co-stimulatory molecule in the murine system

Original languageEnglish
JournalInternational Immunology
Volume7
Issue number4
Pages (from-to)551-557
Number of pages7
ISSN0953-8178
DOIs
Publication statusPublished - 1 Apr 1995
Externally publishedYes

Keywords

  • Armenian hamster
  • CD27
  • Co-stimulation
  • MAb generation
  • Murine T cells
  • Tumor necrosis factor receptor family

Fingerprint

Dive into the research topics of 'Novel mAbs reveal potent co-stimulatory activity of murine CD27'. Together they form a unique fingerprint.

Cite this