Occupational COPD and HMOX1 repeats in a Danish population

Else Würtz, Charlotte Brasch-Andersen, Rudi Steffensen, Jens Georg Hansen, Tine H. Malling, Øyvind Omland, Vivi Schlünssen

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Abstract

Background: Dinucleotide repeats (GT)n in the 5’prime promoter region of the heme oxygenase 1 (HMOX1) gene modulate the gene expression. Long repeats might affect occurrence of COPD. We aimed to investigate associations of the HMOX1 polymorphism of (GT)n repeats to occurrence of COPD.Methods: This population based cohort included 4703 Danes aged 45-84 of Northern European descents. COPD was defined by LLN: 2.5th FEV1/FVC and FEV1 centiles. The occupational exposures were defined as years with vapour, gas, dust or fume (VGDF) exposure. The HMOX1 repeat was genotyped by fragment analysis and capillary electrophoresis and grouped according to short (S): ≤26, medium (M): 27-32 and long (L): ≥33 GT repeat alleles in an L dominant genetic model. Associations were analysed with mixed random effect logistic regression adjusted for smoking, sex, occupational exposure, age and general practitioner practice. Currently, analyses are attempted replicated in a younger Danish cohort aged 20-44.Results: A HMOX1 (GT)n genotype was present in 4423 participants and distributed as S/S 12%, S/M 42%, M/M 35%, S/L 4%, M/L 7% and L/L 0.1%. The crude association between COPD and at least one long GT repeat (S/L, M/L, L/L) GT genotype was significant, p<0.01. After adjustment, parts of all variables were significantly associated to COPD. The odds ratio for the HMOX1 L dominant variable and COPD was 1.74 (95% CI: 1.17-2.59) and for 5-14 years VGDF exposure and COPD OR 1.62 (95% CI: 1.02-2.56).Conclusion: The study supports an association between occupational exposure and COPD with HMOX1 repeats in the model. The association of a long GT repeat in HMOX1 seems to be comparable to a medium occupational VGDF exposure.
Original languageEnglish
Article numberPA418
JournalEuropean Respiratory Journal
Volume50
Issue numbersuppl. 61
ISSN0903-1936
Publication statusPublished - 1 Sep 2017
EventERS International Congress 2017 - Milan, Italy
Duration: 9 Sep 201713 Sep 2017
https://erscongress.org/home2017.html

Conference

ConferenceERS International Congress 2017
CountryItaly
CityMilan
Period09/09/201713/09/2017
Internet address

Cite this

Würtz, Else ; Brasch-Andersen, Charlotte ; Steffensen, Rudi ; Hansen, Jens Georg ; Malling, Tine H. ; Omland, Øyvind ; Schlünssen, Vivi. / Occupational COPD and HMOX1 repeats in a Danish population. In: European Respiratory Journal. 2017 ; Vol. 50, No. suppl. 61.
@article{2759f4e01bed41d6a6849e9de34660ca,
title = "Occupational COPD and HMOX1 repeats in a Danish population",
abstract = "Background: Dinucleotide repeats (GT)n in the 5’prime promoter region of the heme oxygenase 1 (HMOX1) gene modulate the gene expression. Long repeats might affect occurrence of COPD. We aimed to investigate associations of the HMOX1 polymorphism of (GT)n repeats to occurrence of COPD.Methods: This population based cohort included 4703 Danes aged 45-84 of Northern European descents. COPD was defined by LLN: 2.5th FEV1/FVC and FEV1 centiles. The occupational exposures were defined as years with vapour, gas, dust or fume (VGDF) exposure. The HMOX1 repeat was genotyped by fragment analysis and capillary electrophoresis and grouped according to short (S): ≤26, medium (M): 27-32 and long (L): ≥33 GT repeat alleles in an L dominant genetic model. Associations were analysed with mixed random effect logistic regression adjusted for smoking, sex, occupational exposure, age and general practitioner practice. Currently, analyses are attempted replicated in a younger Danish cohort aged 20-44.Results: A HMOX1 (GT)n genotype was present in 4423 participants and distributed as S/S 12{\%}, S/M 42{\%}, M/M 35{\%}, S/L 4{\%}, M/L 7{\%} and L/L 0.1{\%}. The crude association between COPD and at least one long GT repeat (S/L, M/L, L/L) GT genotype was significant, p<0.01. After adjustment, parts of all variables were significantly associated to COPD. The odds ratio for the HMOX1 L dominant variable and COPD was 1.74 (95{\%} CI: 1.17-2.59) and for 5-14 years VGDF exposure and COPD OR 1.62 (95{\%} CI: 1.02-2.56).Conclusion: The study supports an association between occupational exposure and COPD with HMOX1 repeats in the model. The association of a long GT repeat in HMOX1 seems to be comparable to a medium occupational VGDF exposure.",
author = "Else W{\"u}rtz and Charlotte Brasch-Andersen and Rudi Steffensen and Hansen, {Jens Georg} and Malling, {Tine H.} and {\O}yvind Omland and Vivi Schl{\"u}nssen",
year = "2017",
month = "9",
day = "1",
language = "English",
volume = "50",
journal = "European Respiratory Journal",
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Würtz, E, Brasch-Andersen, C, Steffensen, R, Hansen, JG, Malling, TH, Omland, Ø & Schlünssen, V 2017, 'Occupational COPD and HMOX1 repeats in a Danish population', European Respiratory Journal, vol. 50, no. suppl. 61, PA418.

Occupational COPD and HMOX1 repeats in a Danish population. / Würtz, Else; Brasch-Andersen, Charlotte; Steffensen, Rudi; Hansen, Jens Georg; Malling, Tine H.; Omland, Øyvind; Schlünssen, Vivi.

In: European Respiratory Journal, Vol. 50, No. suppl. 61, PA418, 01.09.2017.

Research output: Contribution to journalConference abstract in journalResearchpeer-review

TY - ABST

T1 - Occupational COPD and HMOX1 repeats in a Danish population

AU - Würtz, Else

AU - Brasch-Andersen, Charlotte

AU - Steffensen, Rudi

AU - Hansen, Jens Georg

AU - Malling, Tine H.

AU - Omland, Øyvind

AU - Schlünssen, Vivi

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Background: Dinucleotide repeats (GT)n in the 5’prime promoter region of the heme oxygenase 1 (HMOX1) gene modulate the gene expression. Long repeats might affect occurrence of COPD. We aimed to investigate associations of the HMOX1 polymorphism of (GT)n repeats to occurrence of COPD.Methods: This population based cohort included 4703 Danes aged 45-84 of Northern European descents. COPD was defined by LLN: 2.5th FEV1/FVC and FEV1 centiles. The occupational exposures were defined as years with vapour, gas, dust or fume (VGDF) exposure. The HMOX1 repeat was genotyped by fragment analysis and capillary electrophoresis and grouped according to short (S): ≤26, medium (M): 27-32 and long (L): ≥33 GT repeat alleles in an L dominant genetic model. Associations were analysed with mixed random effect logistic regression adjusted for smoking, sex, occupational exposure, age and general practitioner practice. Currently, analyses are attempted replicated in a younger Danish cohort aged 20-44.Results: A HMOX1 (GT)n genotype was present in 4423 participants and distributed as S/S 12%, S/M 42%, M/M 35%, S/L 4%, M/L 7% and L/L 0.1%. The crude association between COPD and at least one long GT repeat (S/L, M/L, L/L) GT genotype was significant, p<0.01. After adjustment, parts of all variables were significantly associated to COPD. The odds ratio for the HMOX1 L dominant variable and COPD was 1.74 (95% CI: 1.17-2.59) and for 5-14 years VGDF exposure and COPD OR 1.62 (95% CI: 1.02-2.56).Conclusion: The study supports an association between occupational exposure and COPD with HMOX1 repeats in the model. The association of a long GT repeat in HMOX1 seems to be comparable to a medium occupational VGDF exposure.

AB - Background: Dinucleotide repeats (GT)n in the 5’prime promoter region of the heme oxygenase 1 (HMOX1) gene modulate the gene expression. Long repeats might affect occurrence of COPD. We aimed to investigate associations of the HMOX1 polymorphism of (GT)n repeats to occurrence of COPD.Methods: This population based cohort included 4703 Danes aged 45-84 of Northern European descents. COPD was defined by LLN: 2.5th FEV1/FVC and FEV1 centiles. The occupational exposures were defined as years with vapour, gas, dust or fume (VGDF) exposure. The HMOX1 repeat was genotyped by fragment analysis and capillary electrophoresis and grouped according to short (S): ≤26, medium (M): 27-32 and long (L): ≥33 GT repeat alleles in an L dominant genetic model. Associations were analysed with mixed random effect logistic regression adjusted for smoking, sex, occupational exposure, age and general practitioner practice. Currently, analyses are attempted replicated in a younger Danish cohort aged 20-44.Results: A HMOX1 (GT)n genotype was present in 4423 participants and distributed as S/S 12%, S/M 42%, M/M 35%, S/L 4%, M/L 7% and L/L 0.1%. The crude association between COPD and at least one long GT repeat (S/L, M/L, L/L) GT genotype was significant, p<0.01. After adjustment, parts of all variables were significantly associated to COPD. The odds ratio for the HMOX1 L dominant variable and COPD was 1.74 (95% CI: 1.17-2.59) and for 5-14 years VGDF exposure and COPD OR 1.62 (95% CI: 1.02-2.56).Conclusion: The study supports an association between occupational exposure and COPD with HMOX1 repeats in the model. The association of a long GT repeat in HMOX1 seems to be comparable to a medium occupational VGDF exposure.

M3 - Conference abstract in journal

VL - 50

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - suppl. 61

M1 - PA418

ER -