Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Mantas Sereika, Rasmus Hansen Kirkegaard, Søren Michael Karst, Thomas Yssing Michaelsen, Emil Aarre Sørensen, Rasmus Dam Wollenberg, Mads Albertsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleCommunication

88 Citations (Scopus)
46 Downloads (Pure)

Abstract

Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing.
Original languageEnglish
JournalNature Methods
Volume19
Issue number7
Pages (from-to)823-826
Number of pages4
ISSN1548-7091
DOIs
Publication statusPublished - 4 Jul 2022

Bibliographical note

© 2022. The Author(s).

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