Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers

Odete Goncalves, Maria-Jose Banuls, Rafael Alonso, Pilar Jimenez-Meneses, Angel Maquieira, Henrik Vorum, Steffen B. Petersen, Maria Teresa Neves-Petersen

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Abstract

In today's point-of-care testing (POCT), there is an ever-increasing demand for novel and more efficient devices for early diagnosis, especially in cardiovascular diseases (CVD). Early detection of CVD markers, such as Troponin present in the bloodstream, is a key factor for reducing CVD mortality rates. Thiol-ene coupling (TEC) and Light Assisted Molecular Immobilization (LAMI) are photonic techniques leading to immobilization of bioreceptors, such as, antibodies which recognize cardiac markers. These techniques present advantages compared to traditional immobilization techniques since, e.g., there are no thermal or chemical steps and they work in water media. TEC reaction takes place at close-to-visible wavelengths (lambda=365nm) which induces the formation of thiol radicals which bind to alkene functional group on the surface through a thioether bond. LAMI secures molecular immobilizations in a spatially oriented, localized and covalent coupling of biomolecules onto thiol reactive surfaces down to submicrometer spatial resolution. LAMI is possible due to a conserved structural motif in proteins: the spatial proximity between aromatic residues and disulfide bridges. When aromatic residues are excited with UV light (275-295nm), disulphide bridges are disrupted and free thiol groups are formed that can bind covalently to a surface decorated with thiol groups. We have achieved successful immobilization of anti-troponin and anti-myoglobin antibodies with both photonic immobilization techniques. The microarrays of immobilized monoclonal antibodies have successfully detected the CVD biomarkers troponin I and myoglobin, as confirmed by fluorescence imaging. A sandwich immunoassay was carried out, Troponin I and Myoglobin were detected down to 10 ng/mL and 1 ng/mL, respectively.
Original languageUndefined/Unknown
Title of host publicationBiophotonics : Photonic Solutions for Better Health Care VI
EditorsJ Popp, VV Tuchin, FS Pavone
Number of pages15
Volume10685
PublisherSPIE - International Society for Optical Engineering
Publication date2018
Article number106852Z-1
ISBN (Print)978-1-5106-1897-8
DOIs
Publication statusPublished - 2018
EventConference on Biophotonics: Photonic Solutions for Better Health Care - Strasbourg, France
Duration: 23 Apr 201826 Apr 2018
Conference number: VI

Conference

ConferenceConference on Biophotonics
NumberVI
CountryFrance
CityStrasbourg
Period23/04/201826/04/2018
SeriesProceedings of the Society of Photo-optical Instrumentation Engineers (SPIE)
Volume10685
ISSN0277-786X

Cite this

Goncalves, O., Banuls, M-J., Alonso, R., Jimenez-Meneses, P., Maquieira, A., Vorum, H., Petersen, S. B., & Neves-Petersen, M. T. (2018). Photonic immobilization techniques used for the detection of cardiovascular disease biomarkers. In J. Popp, VV. Tuchin, & FS. Pavone (Eds.), Biophotonics: Photonic Solutions for Better Health Care VI (Vol. 10685). [106852Z-1] SPIE - International Society for Optical Engineering. Proceedings of the Society of Photo-optical Instrumentation Engineers (SPIE), Vol.. 10685 https://doi.org/10.1117/12.2305717