Polymer films with size-selected silver nanoparticles as plasmon resonance-based transducers for protein sensing

Hanif Muhammad, Raghavendra Rao Juluri, Peter Fojan, Vladimir Popok

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Transducers based on the phenomenon of localised surface plasmon resonance (LSPR) are fabricated using silver nanoparticles (NPs) imbedded into thin poly (methyl methacrylate) films. The particles are produced by magnetron sputtering, size selected with a precision ≤ 10% of the diameter and deposited on the films in vacuum. Immersion of NPs is controlled by post-deposition thermal annealing providing very good adhesion, in particular, resistance against following wet chemical procedures. LSPR properties of silver NPs are exploited for protein detection using a classical antibody-antigen scheme through covalent attachment of antibody molecules to the particles, followed by incubation with antigen (chicken egg albumin). Atomic force microscopy studies reveal that antibodies are predominantly attached to NPs providing binding sites for the antigens of interest. Specific changes in the LSPR band are observed for each step of protein incubation on NPs. Thus, the transducers demonstrate good sensitivity and selectivity in detection. Flexibility of polymer substrates paves a way for making devices of required configuration.
    Original languageEnglish
    JournalBiointerface Research in Applied Chemistry
    Volume6
    Issue number5
    Pages (from-to)1564-1568
    ISSN2069-5837
    Publication statusPublished - 20 Oct 2016

    Fingerprint

    Transducers
    Silver
    Polymer films
    Nanoparticles
    Polymers
    Surface Plasmon Resonance
    Surface plasmon resonance
    Proteins
    Antigens
    Antibodies
    Atomic Force Microscopy
    Immersion
    Polymethyl Methacrylate
    Vacuum
    Magnetron sputtering
    Ovum
    Albumins
    Chickens
    Atomic force microscopy
    Adhesion

    Keywords

    • silver nanoparticles
    • polymer films
    • localised surface plasmon resonance
    • optical transducers
    • protein sensing

    Cite this

    @article{95e5133c4f8a492099b3116beb9fb8b0,
    title = "Polymer films with size-selected silver nanoparticles as plasmon resonance-based transducers for protein sensing",
    abstract = "Transducers based on the phenomenon of localised surface plasmon resonance (LSPR) are fabricated using silver nanoparticles (NPs) imbedded into thin poly (methyl methacrylate) films. The particles are produced by magnetron sputtering, size selected with a precision ≤ 10{\%} of the diameter and deposited on the films in vacuum. Immersion of NPs is controlled by post-deposition thermal annealing providing very good adhesion, in particular, resistance against following wet chemical procedures. LSPR properties of silver NPs are exploited for protein detection using a classical antibody-antigen scheme through covalent attachment of antibody molecules to the particles, followed by incubation with antigen (chicken egg albumin). Atomic force microscopy studies reveal that antibodies are predominantly attached to NPs providing binding sites for the antigens of interest. Specific changes in the LSPR band are observed for each step of protein incubation on NPs. Thus, the transducers demonstrate good sensitivity and selectivity in detection. Flexibility of polymer substrates paves a way for making devices of required configuration.",
    keywords = "silver nanoparticles, polymer films, localised surface plasmon resonance, optical transducers, protein sensing",
    author = "Hanif Muhammad and Juluri, {Raghavendra Rao} and Peter Fojan and Vladimir Popok",
    year = "2016",
    month = "10",
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    language = "English",
    volume = "6",
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    journal = "Biointerface Research in Applied Chemistry",
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    Polymer films with size-selected silver nanoparticles as plasmon resonance-based transducers for protein sensing. / Muhammad, Hanif; Juluri, Raghavendra Rao; Fojan, Peter; Popok, Vladimir.

    In: Biointerface Research in Applied Chemistry, Vol. 6, No. 5, 20.10.2016, p. 1564-1568.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Polymer films with size-selected silver nanoparticles as plasmon resonance-based transducers for protein sensing

    AU - Muhammad, Hanif

    AU - Juluri, Raghavendra Rao

    AU - Fojan, Peter

    AU - Popok, Vladimir

    PY - 2016/10/20

    Y1 - 2016/10/20

    N2 - Transducers based on the phenomenon of localised surface plasmon resonance (LSPR) are fabricated using silver nanoparticles (NPs) imbedded into thin poly (methyl methacrylate) films. The particles are produced by magnetron sputtering, size selected with a precision ≤ 10% of the diameter and deposited on the films in vacuum. Immersion of NPs is controlled by post-deposition thermal annealing providing very good adhesion, in particular, resistance against following wet chemical procedures. LSPR properties of silver NPs are exploited for protein detection using a classical antibody-antigen scheme through covalent attachment of antibody molecules to the particles, followed by incubation with antigen (chicken egg albumin). Atomic force microscopy studies reveal that antibodies are predominantly attached to NPs providing binding sites for the antigens of interest. Specific changes in the LSPR band are observed for each step of protein incubation on NPs. Thus, the transducers demonstrate good sensitivity and selectivity in detection. Flexibility of polymer substrates paves a way for making devices of required configuration.

    AB - Transducers based on the phenomenon of localised surface plasmon resonance (LSPR) are fabricated using silver nanoparticles (NPs) imbedded into thin poly (methyl methacrylate) films. The particles are produced by magnetron sputtering, size selected with a precision ≤ 10% of the diameter and deposited on the films in vacuum. Immersion of NPs is controlled by post-deposition thermal annealing providing very good adhesion, in particular, resistance against following wet chemical procedures. LSPR properties of silver NPs are exploited for protein detection using a classical antibody-antigen scheme through covalent attachment of antibody molecules to the particles, followed by incubation with antigen (chicken egg albumin). Atomic force microscopy studies reveal that antibodies are predominantly attached to NPs providing binding sites for the antigens of interest. Specific changes in the LSPR band are observed for each step of protein incubation on NPs. Thus, the transducers demonstrate good sensitivity and selectivity in detection. Flexibility of polymer substrates paves a way for making devices of required configuration.

    KW - silver nanoparticles

    KW - polymer films

    KW - localised surface plasmon resonance

    KW - optical transducers

    KW - protein sensing

    UR - http://biointerfaceresearch.com/?download=1626

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    VL - 6

    SP - 1564

    EP - 1568

    JO - Biointerface Research in Applied Chemistry

    JF - Biointerface Research in Applied Chemistry

    SN - 2069-5837

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