Potency assays for human adipose-derived stem cells as a medicinal product toward wound healing

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Abstract

In pre-clinical studies, human adipose-derived stem cells (hASCs) have shown great promise as a treatment modality for healing of cutaneous wounds. The advantages of hASCs are that they are relatively easy to obtain in large numbers from basic liposuctions, they maintain their characteristics after long-term in vitro culture, and they possess low immunogenicity, which enables the use of hASCs from random donors. It has been hypothesized that hASCs exert their wound healing properties by reducing inflammation, inducing angiogenesis, and promoting fibroblast and keratinocyte growth. Due to the inherent variability associated with the donor-dependent nature of ASC-based products, it appears necessary that the quality of the different products is prospectively certified using a set of most relevant potency assays. In this review, we present an overview of the available methodologies to assess the Mode and the Mechanism of Action of hASCs, specifically in the wound healing scenario. In conclusion, we propose a panel of potential potency assays to include in the future production of ASC-based medicinal products.

Original languageEnglish
Article number249
JournalStem Cell Research & Therapy
Volume13
Issue number1
Pages (from-to)249
ISSN1757-6512
DOIs
Publication statusPublished - 11 Jun 2022

Bibliographical note

© 2022. The Author(s).

Keywords

  • Adipose-derived stem cells
  • Chronic wounds
  • Mechanism of action
  • Mode of action
  • Potency assay
  • Stem cell-based medicinal product
  • Wound healing

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